FAT4 Human Gene Knockout Kit (CRISPR)

CAT#: KN220240

FAT4 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: FAT4
• Locus ID: 79633
• Components:

  KN220240G1, FAT4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GATACTGATAGAGTGTGCAA

  KN220240G2, FAT4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACAGTAGCCAAAACACTCGA

  KN220240D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TGGAGGGGCG TTGCAGCCTG GTTTTTGAGG AGTGGGGACT CCAGGAATTC CATCGCCTCC AGCTTTTGGG AAAGAAATTC GATTCTCCCG GTTCCCCACC ACCCCTTCCC CGGTGCGCAG TTGTGCTTGG ACGTTTGTTC CTCCCTCTTC ACGTTCTTCG CTGCGGGTAA GTTCTAAAGT TTCTGAAGAC CGTTCTTTGC AATGATTCCT CATATACCTT AGATACAGGC AACTTCTCCC AACTCTCATC CACCCGGGTG AAAACGCTCA GACTATCTGG ATTCAAAAAC AAAGTAAAAG GGGGCATATA TAAGAGGCTT GAGAAACTTT TCTGGGAACT CAGCTCACAG GAGTGTCCCG CGGAATGCCC TGCCGCTTTT CGCCACAGCA TCTCTCTTGC ACTCCGCGTT CAACTGGCTA CCTAGAGTCT TTTGCTGATG CTACTTGCTT TTGCCGGACT GGAGGTTCTT TGAAATAGCA GAGGTCTCAG ACCAAGCCGT CAGCTGAATC TTTGCTGGCG CTCCTTAATC CCTGTAAATA TCATTGCGTT TGCTTCACCC CTTCCTTCTC TTTATCACAT CGTTTTAGGG AGCCAGGACC AGGCCTGGGT CCACGGGGCC GAGCCGCGCC AGGTGTTCCA AGTGCTGGAA GAGCAACCTC CAGGCACTCT GGTAGGCACC ATCCAGACGC GCCCCGGCTT CACCTACAGG CTCAGCGAAA GCCACGCCCT GTTTGCCATA AACAGTAGCA CCGGAGCCCT GTACACCACC TCCACCATCG ACCGCGAGAG CCTGCCCAGC GACGTGATCA ACCTGGTGGT CCTTTCCAGC GCGCCCACCT ACCCCACCGA AGTGCGAGTG CTGGTGCGGG ACCTCAATGA CAACGCCCCC GTTTTCCCGG ACCCCTCTAT CGTGGTCACT TTCAAGGAAG ACAGTAGCAG CGGACGCCAA GTCATCTTAG ACACCGCCAC CGACTCGGAC ATCGGCTCAA ACGGTGTGGA CCACCGCTCC TACCGCATCA TCCGCGGCAA TGAGGCGGGG CGCTTCCGTC TGGACATCAC CCTGAACCCG AGCGGCGAGG GAGCGTTCCT GCATCTGGTG TCCAAGGGCG GACTGGACCG TGAGGTCACT CCGCAGTACC AGCTCCTGGT TGAGGTGGAG GACAAGGGTG AGCCTAAGCG GCGGGGCTAC CTTCAGGTAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001291285, NM_001291303, NM_024582
• UniProt ID: Q6V0I7
• Synonyms: CDHF14; CDHR11; FAT-J; FATJ; HKLLS2; NBLA00548; VMLDS2
• Summary: The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]