ARK5 (NUAK1) Human Gene Knockout Kit (CRISPR)
CAT#: KN219282RB
NUAK1 - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
USD 1,657.00
4 Weeks*
Specifications
Product Data | |
Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
Donor DNA | RFP-BSD |
Symbol | ARK5 |
Locus ID | 9891 |
Components |
KN219282G1, ARK5 gRNA vector 1 in pCas-Guide CRISPR vector KN219282G2, ARK5 gRNA vector 2 in pCas-Guide CRISPR vector KN219282RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
Reference Data | |
RefSeq | NM_014840 |
UniProt ID | O60285 |
Synonyms | ARK5 |
Summary | Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
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KN219282 | NUAK1 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN219282BN | NUAK1 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN219282LP | NUAK1 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN419282 | NUAK1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. |
USD 1,657.00 |
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GA106678 | NUAK1 CRISPRa kit - CRISPR gene activation of human NUAK family kinase 1 |
USD 1,657.00 |
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