NG2 (CSPG4) Human Gene Knockout Kit (CRISPR)

CAT#: KN218462RB

CSPG4 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: NG2
• Locus ID: 1464
• Components:

  KN218462G1, NG2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN218462G2, NG2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN218462RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001897
• UniProt ID: Q6UVK1
• Synonyms: HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
• Summary: A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]