TCF4 Human Gene Knockout Kit (CRISPR)
CAT#: KN217718
TCF4 - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: Luciferase-Puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
USD 1,657.00
4 Weeks*
Specifications
Product Data | |
Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
Donor DNA | GFP-puro |
Symbol | TCF4 |
Locus ID | 6925 |
Components |
KN217718G1, TCF4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAAAGAGGAGAGGCACCTG KN217718G2, TCF4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTTGACAAATTACTCAAAGA KN217718D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences: pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGCTGTGTAT TAGAATTACT TGGGGAGATT TTTTAAAATC TCAATGCCCA AGCCACACTC CTAGACCAAT TAATTTAGAA GTTCTTAGGA TGAGACTGAG ACATCGTTTT TTTTCTTTTT TCTTTCTTTC TTTCTTTTTT TTTTTAACCA TCCCAAGGTC TTTATTTATT TAGTTTATGC CTATCATGCC ATGAATTCAT GGGGAATAGA TTCTAGCAGC TCGAGCTCCT TTTCTTTGGG TCTCAGAAAA TGTGCTTCTC TGGGCAGAGC AGGCTGGTTT TTCGGTTGAA CCTAGATCCC TTTCTCTTCG GCTTCCTTCT TTTTCTGATC ATTTTCCTCC TACCATTTCA GGATGCTATC TCAGCTCTTA GAGTGCTTAA TATGCTCAAT ATGTACATTA ATTCTCTTGG CAAGAATCTT TCTCTTATCT TGTTTGTTTA CAACAATGCC AACAGCATGC TGGGTTACAC AGTAGACTCT TCCAGCTTTG CCATGGTAAC ACTTGTGCAG CATTCCTTTT CGAACAGTGC CCATTCTCTT GATGTCTACA GTATCACCTT TCTTGTAGAT TGGCACTTAT GCGGCCAAAG GAACAACACC AAAGCCAGCC ATCATTTTTT AAAACTTCTC AGGACATTGT AACATGCACT TGGGTTGAGA ACTGCTACTC GAGCTTCTCC AGGAGGCCCT TGGAGGTAGG AACCAGCATT TGGGCTACTT TAGCATCTTA GTAGAAACTG AAACCAAAAA TGATCTCCCA TGGCCACCAT GGCCAAACAT AACGGTAGTT TCTAGCTCTC TAACCTCTCT CCCTGACATC TCCACAGCAC TTGGCCTGCC CTCTTGAGAG TCTTTTCTTT TTTTCTTTTT CTTTTCTTTT TTTTTTTTTG AGACAGGGCC TCACTCTGTC TCACAGGCTG GAGTGCAATG GCACATTCTT GGCTCACTGC AGCCTCTGCC TCCCGGGTTC TAGCAATTCT CATGCCTCAG CCTCCCAAGT AGCTGAGATT ACAGGCATGC ACCACAATGC CCGGCTAACT TCTGTATTTT TAGTAGAGAC AGGGTTTTGC CATGTTGGTT GGGCTGGCCT CGAATTCCTG ACTTCAAGTG ATCTGTCCAT CTCGGCCTCC CAAAGTGCTG GGATTACAGG CATGAGCCAC CACGCATGGC CCTGCCTTCT TGAGATTCTT GCTGAGACTGGE100003, scramble sequence in pCas-Guide vector |
Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
Reference Data | |
RefSeq | NM_001083962, NM_001243226, NM_001243227, NM_001243228, NM_001243230, NM_001243231, NM_001243232, NM_001243233, NM_001243234, NM_001243235, NM_001243236, NM_003199, NM_001306207, NM_001306208, NM_001330604, NM_001330605, NM_001348211, NM_001348212, NM_001348213, NM_001348214, NM_001348215, NM_001348216, NM_001348217, NM_001348218, NM_001348219, NM_001348220, NM_001369567, NM_001369571, NM_001369574, NM_001369577, NM_001369579, NM_001369580, NM_001369581, NM_001369585, NM_001369586, NM_001369568, NM_001369569, NM_001369570, NM_001369572, NM_001369573, NM_001369575, NM_001369576, NM_001369578, NM_001369582, NM_001369583, NM_001369584 |
UniProt ID | P15884 |
Synonyms | bHLHb19; E2-2; ITF-2; ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4 |
Summary | This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
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KN217718BN | TCF4 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN217718LP | TCF4 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN217718RB | TCF4 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN417718 | TCF4 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. |
USD 1,657.00 |
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GA104778 | TCF4 CRISPRa kit - CRISPR gene activation of human transcription factor 4 |
USD 1,657.00 |
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