HIF1 beta (ARNT) Human Gene Knockout Kit (CRISPR)

CAT#: KN216724LP

ARNT - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: HIF1 beta
• Locus ID: 405
• Components:

  KN216724G1, HIF1 beta gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACTACTGCCAACCCCGGTG

  KN216724G2, HIF1 beta gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGACTGGGGCGCCTCTAAA

  KN216724LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  GCTAATTTTG TATTTTTAGT AGAGACGGGG TTTCGCCATG TTGGCCAGGC TGGTTTCGAA CCCCTGGCCA CAGGTGATCC GGCCGCCTCG GCCTCCCAAA GTGCTGGGAT TACAGACGCG AGCCACCGCG CCCAGCCTAA AATACCCATT TTAATTGCAT TTCAATGACG TTACTCGGTC ATTGACCTCT TACGCAAGGA GGGCGAGATT AGGGAGACAG CTGGACTTCT TCCTCGCCCT CCCTTCACTG GACTGGCTGG CGCAGTGAGT AGCCCTGCCC TTGGACGATT TGGCATTTTC ATTGGTCAAT TTTTCTTAGG AGGCTGGCCG TGTGTTGACT CCGCCTACTA TATAGGCGGG GTCTCCCCGC CGCAGGGGCT GGGATGCTGG GGGCTGCTGA AGCCGCCATC TTGGATTCCG CGGTAGCGGA GGCGGCGGTC AGGCGCCGCT TCTGGGGAGT GGCCTTTCTT TTCCCCTCCC TCCCGGTTCG GTGGCGGCGG CTCCTCCCAC TGGGGGGGGG GGTGGCGCGG CGGCGGTGGC ATCTGCGGCC TGAGGGGACG CAGCTGGAGC TGAAGGCGCC GGGGCGGGGC GGGGGCCGCG AGAGACGGTG GGGGAGCGGT GACCTGGGGA GGGGCCCGAT CCCGGGGACT TGGGTAGACT CGAGTATACC CAAGGGAGCG AGGGGGCAGG TGGGAGTTGA GGGGGGCTCC CTGTCCTGAC CTCCGTTGGA GCGGCAGCCA GTGCGGGGCT GGCCCGCCCT CACTCCTCGG TCGGCGTCTG GTCTGTGATG TGGTCCTCTT TTAACCCTCT GCCCGCTGGA GCCGCTTGAT GCCGAAAACT GTAGGCAGAG TGTCTCGGTT TACTTTAAGA GTAGTTTGGA TTCATAAACC CTGGACCACA CTGCTCTTTA GTTGAGGCGT TGCTTCCAAA CATTTAAAGG TTTAGATTTT AATTTAAAAA ATAAAAAATG GACATTCTTT GATATATACG ACGTTTCTTT AGGCTGTTAC CTCCCACCCG CAACCCCCGT CCCCGTCTCC GTCCCCGGAC TATACTTTGA AACTTAGATC TTGTGTTAAG AGGGCCTGTA GGTCTTTGCC TGCTGAGGGC AGGGGTTTGT GTAATGGTTA TGGTTCCGGA CAGGTCTTTA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001197325, NM_001286035, NM_001286036, NM_001668, NM_178426, NM_178427, NM_001350224, NM_001350225, NM_001350226
• UniProt ID: P27540
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Summary: This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]