SBF2 Human Gene Knockout Kit (CRISPR)

CAT#: KN213601LP

SBF2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: SBF2
• Locus ID: 81846
• Components:

  KN213601G1, SBF2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN213601G2, SBF2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN213601LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  ATTCCCATCT ACACAACATA AACAGGAAAC GTGCAGCAAA AGCCCTGTCG ATGCAAATTC CTTGCTTCCA ACCGAGGGCG GGAGAATTTC ATCTTTGGTG GTGGTGGGGA GCAGAGGAAG CAGCCAGCTT TCCAAGTGAC CCCTCAACTC TCCACATCCC TTCTTCCAAA TCCAAACAGA ATAGAGAAGG CGGAAGGAAG GACCCCCTGC TTTAGGAAAA ATAGGGAGGC AGAGGGGGCC GTGAAGCAGC ATCCAGGCCT CAAAGTGAAC CACCTCCAGG GGCCGCGGTG GCGGCGCGTG GCGGGAACAC GCCCGACCGC CCACTCCCCC AGCGCAGCAG TCCGCGAGGC CCGCGGAGGG TGGAGGTGGG CTCGCCGCCC GGGCTTGGGG CAGCCGAGCC AGCGCGGGTG GGGGCGGGGG AGGAGCGGGC ACTAGGCGCC GCGCAAGCGC AGAGCGCGCC TCGGTTGTCA AACATGGCTG AAGCGCTGCC GCTGCCGCTA CTGCCGCTGC AGGGAAAATG CTGAGCCCTC CCGGGCCGGG TGGGCGGCGG CGGCGAGGGC GGCGACGGGG ACCCGCTTCC CGAGCGCGGC GGCGGCGGCC GCCTCCCCGC CCCCCGGCCG CTGTCCAGTG GGCTCCGCGG GGCCGGGCGT CGGGGACGGG CGTCGGGGAG GGCCAGGCCG AGTCTCGGAG GAGCGGGCGG TCGGCGTCCG GCCTTCTCGG CTGGGCTGAG CCTGGCGGTG GGCGTGGGGG GGCCGGCACG ACGGTAGGGG GAGGGAAGAC GCCGCGTCTG CCCAGCCCCT GGGCGCACCC GCTGGGCGGG CCGGAGGCGA GTTGGGGTCT CGCGACCCTC CCGCTGGAGG CTGCAGGGTA CCCGGGGCAG AGAGAGCCCT GAGATTGGGC CCAGGACCGC AAGGCTGGGA GGGCGGGAGC CAACTGTGCT AAGTTCCTCG GGGATAAACT TCTGCCCTGC ATCCTGAGTG AGTAACCAGT GACGCAGGGG GTTGTCCTCT CCGAAAGGAA GTGCTGAGAG TCCAGTGCTA ACTCACCCCT GTGCTGTTGG GGGCCTAGTG ACCTCCCCAA GGAACGCGTG GTACCAGGGA AGGGGACAGG ATTCTTTGCA CTTTTACCCC TTTCTAGGAA ATGCTCCCTA TTCTTTCAGG ACAAATGTGA CGGTCACTTC ACAGTGACAT GACTTGTCTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_030962
• UniProt ID: Q86WG5
• Synonyms: CMT4B2; DENND7B; MTMR13
• Summary: This gene encodes a pseudophosphatase and member of the myotubularin-related protein family. This gene maps within the CMT4B2 candidate region of chromosome 11p15 and mutations in this gene have been associated with Charcot-Marie-Tooth Disease, type 4B2. [provided by RefSeq, Jul 2008]