Dicer (DICER1) Human Gene Knockout Kit (CRISPR)

CAT#: KN211804BN

DICER1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: Dicer
• Locus ID: 23405
• Components:

  KN211804G1, Dicer gRNA vector 1 in pCas-Guide CRISPR vector

  KN211804G2, Dicer gRNA vector 2 in pCas-Guide CRISPR vector

  KN211804BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001195573, NM_001271282, NM_001291628, NM_030621, NM_177438
• UniProt ID: Q9UPY3
• Synonyms: DCR1; Dicer; Dicer1e; HERNA; K12H4.8-LIKE; MNG1; RMSE2
• Summary: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]