CD73 (NT5E) Human Gene Knockout Kit (CRISPR)

CAT#: KN209568LP

NT5E - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: CD73
• Locus ID: 4907
• Components:

  KN209568G1, CD73 gRNA vector 1 in pCas-Guide CRISPR vector

  KN209568G2, CD73 gRNA vector 2 in pCas-Guide CRISPR vector

  KN209568LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001204813, NM_002526
• UniProt ID: P21589
• Synonyms: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE
• Summary: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]