Occludin (OCLN) Human Gene Knockout Kit (CRISPR)

CAT#: KN206468LP

OCLN - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: Occludin
• Locus ID: 100506658
• Components:

  KN206468G1, Occludin gRNA vector 1 in pCas-Guide CRISPR vector

  KN206468G2, Occludin gRNA vector 2 in pCas-Guide CRISPR vector

  KN206468LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001205254, NM_001205255, NM_002538
• UniProt ID: Q16625
• Synonyms: BLCPMG; PPP1R115
• Summary: This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]