AJUBA Human Gene Knockout Kit (CRISPR)
CAT#: KN205482BN
AJUBA - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro RFP-BSD
HDR-mediated knockout kit validation
USD 1,657.00
4 Weeks*
Specifications
Product Data | |
Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
Donor DNA | mBFP-Neo |
Symbol | AJUBA |
Locus ID | 84962 |
Components |
KN205482G1, AJUBA gRNA vector 1 in pCas-Guide CRISPR vector KN205482G2, AJUBA gRNA vector 2 in pCas-Guide CRISPR vector KN205482BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. GE100003, scramble sequence in pCas-Guide vector |
Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
Reference Data | |
RefSeq | NM_001289097, NM_032876, NM_198086 |
UniProt ID | Q96IF1 |
Synonyms | JUB |
Summary | Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
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KN205482 | AJUBA - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN205482LP | AJUBA - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN205482RB | AJUBA - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
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KN405482 | AJUBA - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. |
USD 1,657.00 |
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GA113804 | AJUBA CRISPRa kit - CRISPR gene activation of human ajuba LIM protein |
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