alpha 1 Antitrypsin (SERPINA1) Human Gene Knockout Kit (CRISPR)

CAT#: KN202082

SERPINA1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: alpha 1 Antitrypsin
• Locus ID: 5265
• Components:

  KN202082G1, alpha 1 Antitrypsin gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ATGCCGTCTTCTGTCTCGTG

  KN202082G2, alpha 1 Antitrypsin gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCCCTGGCTGAGGATCCCCA

  KN202082D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTTCTGTGCA GCAGTTTCCC TGGCCACTAA ATAGTGGGAT TAGATAGAAG CCCTCCAAGG GCTTCCAGCT TGACATGATT CTTGATTCTG ATCTGGCCCG ATTCCTGGAT AATCGTGGGC AGGCCCATTC CTCTTCTTGT GCCTCATTTT CTTCTTTTGT AAAACAATGG CTGTACCATT TGCATCTTAG GGTCATTGCA GATGTAAGTG TTGCTGTCCA GAGCCTGGGT GCAGGACCTA GATGTAGGAT TCTGGTTCTG CTACTTCCTC AGTGACATTG AATAGCTGAC CTAATCTCTC TGGCTTTGGT TTCTTCATCT GTAAAAGAAG GATATTAGCA TTAGCACCTC ACGGGATTGT TACAAGAAAG CAATGAATTA ACACATGTGA GCACGGAGAA CAGTGCTTGG CATATGGTAA GCACTACGTA CATTTTGCTA TTCTTCTGAT TCTTTCAGTG TTACTGATGT CGGCAAGTAC TTGGCACAGG CTGGTTTAAT AATCCCTAGG CACTTCCACG TGGTGTCAAT CCCTGATCAC TGGGAGTCAT CATGTGCCTT GACTCGGGGC CTGGCCCCCC CATCTCTGTC TTGCAGGACA GAAGACAGAT ACATCCCACC ATGATCAGGA TCACCCAACC TTCAACAAGA TCACCCCCAA CCTGGCTGAG TTCGCCTTCA GCCTATACCG CCAGCTGGCA CACCAGTCCA ACAGCACCAA TATCTTCTTC TCCCCAGTGA GCATCGCTAC AGCCTTTGCA ATGCTCTCCC TGGGGACCAA GGCTGACACT CACGATGAAA TCCTGGAGGG CCTGAATTTC AACCTCACGG AGATTCCGGA GGCTCAGATC CATGAAGGCT TCCAGGAACT CCTCCGTACC CTCAACCAGC CAGACAGCCA GCTCCAGCTG ACCACCGGCA ATGGCCTGTT CCTCAGCGAG GGCCTGAAGC TAGTGGATAA GTTTTTGGAG GATGTTAAAA AGTTGTACCA CTCAGAAGCC TTCACTGTCA ACTTCGGGGA CACCGAAGAG GCCAAGAAAC AGATCAACGA TTACGTGGAG AAGGGTACTC AAGGGAAAAT TGTGGATTTG GTCAAGGAGC TTGACAGAGA CACAGTTTTT GCTCTGGTGA ATTACATCTT CTTTAAAGGT AAGGTTGCTC AACCAGCCTG AGCTGTTCCC ATAGAAACAA GCAAAAATAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000295, NM_001002235, NM_001002236, NM_001127700, NM_001127701, NM_001127702, NM_001127703, NM_001127704, NM_001127705, NM_001127706, NM_001127707
• UniProt ID: P01009
• Synonyms: A1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275
• Summary: The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2020]