ZNF274 Human Gene Knockout Kit (CRISPR)

CAT#: KN200209RB

ZNF274 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: ZNF274
• Locus ID: 10782
• Components:

  KN200209G1, ZNF274 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200209G2, ZNF274 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200209RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001278734, NM_001278735, NM_016324, NM_016325, NM_133502
• UniProt ID: Q96GC6
• Synonyms: HFB101; ZF2; ZKSCAN19; ZSCAN51
• Summary: This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008]