Mouse Kiss1 activation kit by CRISPRa

CAT#: GA216189

Kiss1 CRISPRa kit - CRISPR gene activation of mouse KiSS-1 metastasis-suppressor

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Kiss1
• Locus ID: 280287
• Kit Components:

  GA216189G1, Kiss1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA216189G2, Kiss1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA216189G3, Kiss1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_178260
• UniProt ID: Q6Y4S4
• Synonyms: kisspeptin; metastatin
• Summary: Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.[UniProtKB/Swiss-Prot Function]