Mouse A2m activation kit by CRISPRa

CAT#: GA214349

A2m CRISPRa kit - CRISPR gene activation of mouse alpha-2-macroglobulin

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: A2m
• Locus ID: 232345
• Kit Components:

  GA214349G1, A2m gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA214349G2, A2m gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA214349G3, A2m gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_175628
• UniProt ID: Q6GQT1
• Synonyms: A2mp
• Summary: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).[UniProtKB/Swiss-Prot Function]