Mouse Ppp1r16b activation kit by CRISPRa

CAT#: GA214041

Ppp1r16b CRISPRa kit - CRISPR gene activation of mouse protein phosphatase 1, regulatory subunit 16B

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Ppp1r16b
• Locus ID: 228852
• Kit Components:

  GA214041G1, Ppp1r16b gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA214041G2, Ppp1r16b gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA214041G3, Ppp1r16b gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001159662, NM_153089
• UniProt ID: Q8VHQ3
• Synonyms: ANKRD4; C130078N17Rik; TIMAP; Wdt4
• Summary: Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (PubMed:21907835). Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity).[UniProtKB/Swiss-Prot Function]