Mouse Sptssa activation kit by CRISPRa

CAT#: GA211831

Sptssa CRISPRa kit - CRISPR gene activation of mouse serine palmitoyltransferase, small subunit A

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Sptssa
• Locus ID: 104725
• Kit Components:

  GA211831G1, Sptssa gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA211831G2, Sptssa gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA211831G3, Sptssa gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_134054
• UniProt ID: Q8R207
• Synonyms: 1110002B05Rik; AA407909; AA409588; AU041967; Ssspta
• Summary: Stimulates the activity of serine palmitoyltransferase (SPT). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. Plays a role in MBOAT7 location to mitochondria-associated membranes (MAMs), may me involved in fatty acid remodeling phosphatidylinositol (PI).[UniProtKB/Swiss-Prot Function]