Mouse Dgcr8 activation kit by CRISPRa
CAT#: GA211435
Dgcr8 CRISPRa kit - CRISPR gene activation of mouse DGCR8, microprocessor complex subunit
Find the corresponding CRISPRi Inhibitor Kit
USD 1,657.00
2 Weeks*
Specifications
Product Data | |
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Symbol | Dgcr8 |
Locus ID | 94223 |
Kit Components | GA211435G1, Dgcr8 gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA211435G2, Dgcr8 gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA211435G3, Dgcr8 gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077 |
Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc. |
Reference Data | |
RefSeq | NM_033324 |
UniProt ID | Q9EQM6 |
Synonyms | D16H22S788E; D16H22S1742E; D16Wis2; Gy1; mir-1306; N41; Vo59c07 |
Summary | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983).[UniProtKB/Swiss-Prot Function] |
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Other Versions
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KN504526 | Dgcr8 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
USD 1,657.00 |
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