Mouse Tex19.1 activation kit by CRISPRa

CAT#: GA209577

Tex19.1 CRISPRa kit - CRISPR gene activation of mouse testis expressed gene 19.1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Tex19.1
• Locus ID: 73679
• Kit Components:

  GA209577G1, Tex19.1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA209577G2, Tex19.1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA209577G3, Tex19.1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_028602
• UniProt ID: Q99MV2
• Synonyms: 2410081M02Rik; mTex19.1; Tex19; Tex19a
• Summary: Required during spermatogenesis and placenta development, participating in the repression of retrotransposable elements and preventing their mobilization (PubMed:18802469, PubMed:21103378, PubMed:23364048, PubMed:23674551, PubMed:28254886). With its paralog, Tex19.2, collaborates with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Interacts with Piwi proteins and directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:28254886). Also during spermatogenesis, promotes, with UBR2, SPO11-dependent meiotic recombination (PubMed:28708824). Interacts with LINE-1 retrotransposon encoded LIRE1, stimulates LIRE1 polyubiquitination, mediated by UBR2, and degradation, inhibiting LINE-1 retranstoposon mobilization (PubMed:28806172).[UniProtKB/Swiss-Prot Function]