Mouse Sdhaf1 activation kit by CRISPRa

CAT#: GA207781

Sdhaf1 CRISPRa kit - CRISPR gene activation of mouse succinate dehydrogenase complex assembly factor 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Sdhaf1
• Locus ID: 68332
• Kit Components:

  GA207781G1, Sdhaf1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA207781G2, Sdhaf1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA207781G3, Sdhaf1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001033140
• UniProt ID: Q3U276
• Synonyms: 0610010E21Rik; AI430885; AW490662; Lyrm8
• Summary: Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit Sdhb of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF3. Contributes to iron-sulfur cluster incorporation into SDHB by binding to SDHB and recruiting the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20.[UniProtKB/Swiss-Prot Function]