Mouse Gid4 activation kit by CRISPRa

CAT#: GA206948

Gid4 CRISPRa kit - CRISPR gene activation of mouse GID complex subunit 4, VID24 homolog

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Gid4
• Locus ID: 66771
• Kit Components:

  GA206948G1, Gid4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA206948G2, Gid4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA206948G3, Gid4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_025757
• UniProt ID: Q9CPY6
• Synonyms: 4933439F18Rik
• Summary: Substrate-recognition subunit of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Binds proteins and peptides with a Pro/N-degron consisting of an unmodified N-terminal Pro followed by a small residue, and has the highest affinity for the peptide Pro-Gly-Leu-Trp. Binds peptides with an N-terminal sequence of the type Pro-[Ala,Gly]-[Leu,Met,Gln,Ser,Tyr]-[Glu,Gly,His,Ser,Val,Trp,Tyr]. Does not bind peptides with an acetylated N-terminal Pro residue.[UniProtKB/Swiss-Prot Function]