Mouse Fbxo6 activation kit by CRISPRa

CAT#: GA205345

Fbxo6 CRISPRa kit - CRISPR gene activation of mouse F-box protein 6

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Fbxo6
• Locus ID: 50762
• Kit Components:

  GA205345G1, Fbxo6 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA205345G2, Fbxo6 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA205345G3, Fbxo6 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001163704, NM_001163705, NM_001163706, NM_001163707, NM_015797
• UniProt ID: Q9QZN4
• Synonyms: AA408845; FBG2; Fbs2; Fbx6b; Fbxo6b
• Summary: Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication (By similarity). Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans.[UniProtKB/Swiss-Prot Function]