Mouse Smc1a activation kit by CRISPRa

CAT#: GA204881

Smc1a CRISPRa kit - CRISPR gene activation of mouse structural maintenance of chromosomes 1A

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Smc1a
• Locus ID: 24061
• Kit Components:

  GA204881G1, Smc1a gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA204881G2, Smc1a gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA204881G3, Smc1a gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_019710
• UniProt ID: Q9CU62
• Synonyms: 5830426I24Rik; mKIAA0178; Sb1.8; SMC-1A; Smc1; Smc1alpha; Smc1l1; Smcb
• Summary: Involved in chromosome cohesion during cell cycle and in DNA repair. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint (By similarity). Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.[UniProtKB/Swiss-Prot Function]