Mouse Sars activation kit by CRISPRa

CAT#: GA203765

Sars CRISPRa kit - CRISPR gene activation of mouse seryl-aminoacyl-tRNA synthetase

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Sars
• Locus ID: 20226
• Kit Components:

  GA203765G1, Sars gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA203765G2, Sars gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA203765G3, Sars gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001204979, NM_011319
• UniProt ID: P26638
• Synonyms: Sars1; serRS; Strs
• Summary: Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.[UniProtKB/Swiss-Prot Function]