Mouse Ppara activation kit by CRISPRa

CAT#: GA203338

Ppara CRISPRa kit - CRISPR gene activation of mouse peroxisome proliferator activated receptor alpha

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Ppara
• Locus ID: 19013
• Kit Components:

  GA203338G1, Ppara gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA203338G2, Ppara gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA203338G3, Ppara gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001113418, NM_011144
• UniProt ID: P23204
• Synonyms: 4933429D07Rik; AW742785; Nr1c1; Ppar; PPAR-alpha; PPARalpha
• Summary: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.[UniProtKB/Swiss-Prot Function]