Mouse Mtf2 activation kit by CRISPRa

CAT#: GA202746

Mtf2 CRISPRa kit - CRISPR gene activation of mouse metal response element binding transcription factor 2

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Mtf2
• Locus ID: 17765
• Kit Components:

  GA202746G1, Mtf2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA202746G2, Mtf2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA202746G3, Mtf2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001253877, NM_001253878, NM_001253879, NM_001253880, NM_013827, NR_045608, NM_001359088, NM_001359089, NR_152817
• UniProt ID: Q02395
• Synonyms: 9230112N11Rik; AA537621; C76717; M96; Pcl2
• Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter.[UniProtKB/Swiss-Prot Function]