Mouse Gtf2i activation kit by CRISPRa

CAT#: GA201775

Gtf2i CRISPRa kit - CRISPR gene activation of mouse general transcription factor II I

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Gtf2i
• Locus ID: 14886
• Kit Components:

  GA201775G1, Gtf2i gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA201775G2, Gtf2i gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA201775G3, Gtf2i gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001080746, NM_001080747, NM_001080748, NM_001080749, NM_010365, NM_001359062, NM_001359063, NM_001359064, NM_001359065, NM_001359066, NM_001359067
• UniProt ID: Q9ESZ8
• Synonyms: 6030441I21Rik; BAP-135; Diws1t; GtfII-I; Spin; TFII-I; WBSCR6
• Summary: Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.[UniProtKB/Swiss-Prot Function]