Mouse Gabrb3 activation kit by CRISPRa

CAT#: GA201520

Gabrb3 CRISPRa kit - CRISPR gene activation of mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Gabrb3
• Locus ID: 14402
• Kit Components:

  GA201520G1, Gabrb3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA201520G2, Gabrb3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA201520G3, Gabrb3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001038701, NM_008071
• UniProt ID: P63080
• Synonyms: A230092K12Rik; AW049585; beta3; Cp1; Gabrb-3
• Summary: Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447).[UniProtKB/Swiss-Prot Function]