Mouse Atp5k activation kit by CRISPRa

CAT#: GA200331

Atp5k CRISPRa kit - CRISPR gene activation of mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit E

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Atp5k
• Locus ID: 11958
• Kit Components:

  GA200331G1, Atp5k gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA200331G2, Atp5k gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA200331G3, Atp5k gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_007507
• UniProt ID: Q06185
• Synonyms: 2610008D24Rik; Atp5i; Lfm1
• Summary: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.[UniProtKB/Swiss-Prot Function]