Human RNF166 activation kit by CRISPRa

CAT#: GA114556

RNF166 CRISPRa kit - CRISPR gene activation of human ring finger protein 166

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: RNF166
• Locus ID: 115992
• Kit Components:

  GA114556G1, RNF166 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA114556G2, RNF166 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA114556G3, RNF166 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001171815, NM_001171816, NM_178841
• UniProt ID: Q96A37
• Synonyms: MGC2647; MGC14381
• Summary: E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Plays also a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).[UniProtKB/Swiss-Prot Function]