Human POLR1B activation kit by CRISPRa

CAT#: GA113406

POLR1B CRISPRa kit - CRISPR gene activation of human RNA polymerase I subunit B

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: POLR1B
• Locus ID: 84172
• Kit Components:

  GA113406G1, POLR1B gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA113406G2, POLR1B gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA113406G3, POLR1B gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001137604, NM_001282772, NM_001282774, NM_001282776, NM_001282777, NM_001282779, NM_019014, NM_032212
• UniProt ID: Q9H9Y6
• Synonyms: RPA2; RPA135; Rpo1-2
• Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]