Human RIC8 (RIC8A) activation kit by CRISPRa

CAT#: GA111876

RIC8A CRISPRa kit - CRISPR gene activation of human RIC8 guanine nucleotide exchange factor A

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: RIC8A
• Locus ID: 60626
• Kit Components:

  GA111876G1, RIC8 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA111876G2, RIC8 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA111876G3, RIC8 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001286134, NM_021932
• UniProt ID: Q9NPQ8
• Synonyms: RIC8
• Summary: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.[UniProtKB/Swiss-Prot Function]