Human SART2 (DSE) activation kit by CRISPRa

CAT#: GA109325

DSE CRISPRa kit - CRISPR gene activation of human dermatan sulfate epimerase

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: DSE
• Locus ID: 29940
• Kit Components:

  GA109325G1, SART2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA109325G2, SART2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA109325G3, SART2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001080976, NM_013352, NM_001322937, NM_001322938, NM_001322939, NM_001322940, NM_001322941, NM_001322943, NM_001322944, NR_136520, NR_136521, NR_136522, NR_136523, NR_136524
• UniProt ID: Q9UL01
• Synonyms: DSEP; DSEPI; EDSMC2; SART-2; SART2
• Summary: The protein encoded by this gene is a tumor-rejection antigen. It is localized to the endoplasmic reticulum and functions to convert D-glucuronic acid to L-iduronic acid during the biosynthesis of dermatan sulfate. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. Mutations in this gene cause inmusculocontractural Ehlers-Danlos syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9, and a paralogous gene exists on chromosome 18. [provided by RefSeq, Apr 2016]