Human NEDD8 activation kit by CRISPRa

CAT#: GA103162

NEDD8 CRISPRa kit - CRISPR gene activation of human NEDD8 ubiquitin like modifier

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: NEDD8
• Locus ID: 4738
• Kit Components:

  GA103162G1, NEDD8 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103162G2, NEDD8 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103162G3, NEDD8 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006156
• UniProt ID: Q15843
• Synonyms: NEDD-8
• Summary: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.[UniProtKB/Swiss-Prot Function]