Human CYP1A1 activation kit by CRISPRa

CAT#: GA101090

CYP1A1 CRISPRa kit - CRISPR gene activation of human cytochrome P450 family 1 subfamily A member 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: CYP1A1
• Locus ID: 1543
• Kit Components:

  GA101090G1, CYP1A1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101090G2, CYP1A1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101090G3, CYP1A1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000499, NM_001319216, NM_001319217
• UniProt ID: P04798
• Synonyms: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• Summary: This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]