Maf1 (NM_026859) Mouse Tagged ORF Clone Lentiviral Particle

CAT#: MR203339L4V

Lenti ORF particles, Maf1 (GFP-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2, 200ul, >10^7 TU/mL

Specifications

Product Data

• Type: Mouse Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: Maf1
• Synonyms: 1110068E11Rik; AU042856
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_026859
• ORF Size: 777 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR203339).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_026859.2, NP_081135.2
• RefSeq Size: 1703 bp
• RefSeq ORF: 777 bp
• Locus ID: 68877
• UniProt ID: Q9D0U6
• Cytogenetics: 15 D3
• Gene Summary: Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (By similarity). Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (PubMed:30110641). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (By similarity).[UniProtKB/Swiss-Prot Function]