NP (NC_006502) Virus Tagged ORF Clone
CAT#: VC102285
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for putative nucleocapsid protein [Infectious salmon anemia virus], codon optimized for human cell expression, YP_145803
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Frequently bought together (3)
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC102285 represents NCBI reference of YP_145803 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACAAGGGCATGACCTATAGCTTCGATGTGAGAGACAATACCCTCGTGGTGAGACGGAGCACCG CAACCAAGAGCGGCATTAAAATTAGCTATCGGGAAGACCGGGGCACCAGCCTCCTGCAGAAGGCCTTCGC TGGGACCGAGGATGAGTTCTGGGTGGAGCTGGACCAGGACGTGTACGTGGACAAGAAGATCCGGGAGTTC CTGGTGGAGGAGAAAATGAAAGACATGAGCACCCGGGTGTCCGGCGCTGTGGCAGCCGCAATCGAAAGAA GCGTCGAGTTCGATAACTTCTCCAAGGAGGCCGCCGCAAACATCGAGATGGCCGGCGTGGATGACGAGGA GGCCGGCGGGTCCGGCCTGGTGGACAACCGGCGGAAGAACAAGGGGGTCTCCAACATGGCCTACAACCTG AGCCTGTTTATCGGCATGGTCTTCCCCGCCCTGACCACCTTCTTCTCCGCCATCCTGAGCGAGGGGGAGA TGTCCATCTGGCAGAACGGCCAGGCCATCATGCGGATCCTGGCCCTCGCCGACGAGGACGGCAAGCGGCA GACCCGGACCGGGGGCCAGCGGGTCGACATGGCCGACGTCACCAAGCTGAACGTGGTGACCGCCAACGGG AAGGTGAAGCAGGTGGAGGTGAACCTGAACGACCTGAAGGCCGCCTTCCGGCAGTCCCGGCCCAAACGGA GCGACTACCGGAAAGGGCAGGGCAGCAAGGCCACCGAGAGCTCCATCAGCAACCAGTGCATGGCCCTCAT CATGAAGAGCGTGCTGTCCGCCGACCAGCTGTTCGCCCCAGGCGTGAAGATGATGCGGACCAACGGCTTC AATGCCAGCTACACCACCCTGGCCGAGGGCGCCAACATCCCAAGCAAGTACCTGCGGCACATGCGGAACT GCGGCGGCGTGGCCCTCGACCTCATGGGCATGAAGCGGATTAAGAACTCCCCCGAGGGCGCCAAGAGCAA GATCTTCAGCATTATCCAGAAAAAGGTCCGGGGCCGGTGCAGAACCGAGGAGCAGCGGCTGCTCACCAGC GCCCTCAAAATCAGCGATGGCGAGAACAAGTTCCAGCGGATCATGGACACCCTCTGCACCAGCTTCCTCA TCGATCCCCCCCGGACCACCAAGTGCTTCATCCCCCCCATCTCCAGCCTGATGATGTACATCCAAGAGGG CAACAGCGTGCTCGCCATGGACTTCATGAAAAATGGGGAGGACGCCTGCAAGATTTGCCGGGAAGCCAAG CTCAAAGTCGGGGTGAACAGCACCTTCACCATGTCCGTGGCAAGAACCTGTGTGGCCGTGAGCATGGTGG CCACCGCCTTTTGTTCCGCCGACATCATCGAGAACGCCGTGCCCGGCTCCGAGCGGTACCGGAGCAACAT CAAGGCCAACACAACCAAGCCCAAGAAGGACTCCACCTACACCATTCAGGGGCTCCGGCTGTCCAATGTG CGGTACGAGGCCCGGCCCGAGACCAGCCAGAGCAACACCGACCGGAGCTGGCAGGTCAACGTCACAGACT CCTTCGGCGGCCTCGCAGTCTTCAATCAGGGGGCCATCCGGGAAATGCTGGGCGACGGCACAAGCGAAAC CACCAGCGTGAACGTGCGGGCTCTCGTGAAGCGGATCCTGAAGAGCGCCAGCGAACGGAGCGCCCGGGCC GTCAAGACCTTCATGGTCGGGGAGCAGGGGAAGAGCGCCATCGTGATTAGCGGCGTGGGCCTGTTCAGCA TTGACTTCGAAGGCGTCGAAGAGGCTGAGCGGATCACCGATATGACCCCCGAGATCGAGTTCGATGAGGA CGATGAGGAGGAGGAAGACATCGACATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102285 representing YP_145803
Red=Cloning sites Green=Tags MADKGMTYSFDVRDNTLVVRRSTATKSGIKISYREDRGTSLLQKAFAGTEDEFWVELDQDVYVDKKIREF LVEEKMKDMSTRVSGAVAAAIERSVEFDNFSKEAAANIEMAGVDDEEAGGSGLVDNRRKNKGVSNMAYNL SLFIGMVFPALTTFFSAILSEGEMSIWQNGQAIMRILALADEDGKRQTRTGGQRVDMADVTKLNVVTANG KVKQVEVNLNDLKAAFRQSRPKRSDYRKGQGSKATESSISNQCMALIMKSVLSADQLFAPGVKMMRTNGF NASYTTLAEGANIPSKYLRHMRNCGGVALDLMGMKRIKNSPEGAKSKIFSIIQKKVRGRCRTEEQRLLTS ALKISDGENKFQRIMDTLCTSFLIDPPRTTKCFIPPISSLMMYIQEGNSVLAMDFMKNGEDACKICREAK LKVGVNSTFTMSVARTCVAVSMVATAFCSADIIENAVPGSERYRSNIKANTTKPKKDSTYTIQGLRLSNV RYEARPETSQSNTDRSWQVNVTDSFGGLAVFNQGAIREMLGDGTSETTSVNVRALVKRILKSASERSARA VKTFMVGEQGKSAIVISGVGLFSIDFEGVEEAERITDMTPEIEFDEDDEEEEDIDI TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_006502 |
ORF Size | 1848 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_006502.1, YP_145803 |
RefSeq ORF | 1848 bp |
Locus ID | 3170816 |
MW | 68.0 kDa |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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