NP (NC_006502) Virus Tagged ORF Clone

CAT#: VC102285

Myc-DDK-tagged ORF clone of viral ORF for putative nucleocapsid protein [Infectious salmon anemia virus], codon optimized for human cell expression, YP_145803

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102285 represents NCBI reference of YP_145803 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACAAGGGCATGACCTATAGCTTCGATGTGAGAGACAATACCCTCGTGGTGAGACGGAGCACCG
  CAACCAAGAGCGGCATTAAAATTAGCTATCGGGAAGACCGGGGCACCAGCCTCCTGCAGAAGGCCTTCGC
  TGGGACCGAGGATGAGTTCTGGGTGGAGCTGGACCAGGACGTGTACGTGGACAAGAAGATCCGGGAGTTC
  CTGGTGGAGGAGAAAATGAAAGACATGAGCACCCGGGTGTCCGGCGCTGTGGCAGCCGCAATCGAAAGAA
  GCGTCGAGTTCGATAACTTCTCCAAGGAGGCCGCCGCAAACATCGAGATGGCCGGCGTGGATGACGAGGA
  GGCCGGCGGGTCCGGCCTGGTGGACAACCGGCGGAAGAACAAGGGGGTCTCCAACATGGCCTACAACCTG
  AGCCTGTTTATCGGCATGGTCTTCCCCGCCCTGACCACCTTCTTCTCCGCCATCCTGAGCGAGGGGGAGA
  TGTCCATCTGGCAGAACGGCCAGGCCATCATGCGGATCCTGGCCCTCGCCGACGAGGACGGCAAGCGGCA
  GACCCGGACCGGGGGCCAGCGGGTCGACATGGCCGACGTCACCAAGCTGAACGTGGTGACCGCCAACGGG
  AAGGTGAAGCAGGTGGAGGTGAACCTGAACGACCTGAAGGCCGCCTTCCGGCAGTCCCGGCCCAAACGGA
  GCGACTACCGGAAAGGGCAGGGCAGCAAGGCCACCGAGAGCTCCATCAGCAACCAGTGCATGGCCCTCAT
  CATGAAGAGCGTGCTGTCCGCCGACCAGCTGTTCGCCCCAGGCGTGAAGATGATGCGGACCAACGGCTTC
  AATGCCAGCTACACCACCCTGGCCGAGGGCGCCAACATCCCAAGCAAGTACCTGCGGCACATGCGGAACT
  GCGGCGGCGTGGCCCTCGACCTCATGGGCATGAAGCGGATTAAGAACTCCCCCGAGGGCGCCAAGAGCAA
  GATCTTCAGCATTATCCAGAAAAAGGTCCGGGGCCGGTGCAGAACCGAGGAGCAGCGGCTGCTCACCAGC
  GCCCTCAAAATCAGCGATGGCGAGAACAAGTTCCAGCGGATCATGGACACCCTCTGCACCAGCTTCCTCA
  TCGATCCCCCCCGGACCACCAAGTGCTTCATCCCCCCCATCTCCAGCCTGATGATGTACATCCAAGAGGG
  CAACAGCGTGCTCGCCATGGACTTCATGAAAAATGGGGAGGACGCCTGCAAGATTTGCCGGGAAGCCAAG
  CTCAAAGTCGGGGTGAACAGCACCTTCACCATGTCCGTGGCAAGAACCTGTGTGGCCGTGAGCATGGTGG
  CCACCGCCTTTTGTTCCGCCGACATCATCGAGAACGCCGTGCCCGGCTCCGAGCGGTACCGGAGCAACAT
  CAAGGCCAACACAACCAAGCCCAAGAAGGACTCCACCTACACCATTCAGGGGCTCCGGCTGTCCAATGTG
  CGGTACGAGGCCCGGCCCGAGACCAGCCAGAGCAACACCGACCGGAGCTGGCAGGTCAACGTCACAGACT
  CCTTCGGCGGCCTCGCAGTCTTCAATCAGGGGGCCATCCGGGAAATGCTGGGCGACGGCACAAGCGAAAC
  CACCAGCGTGAACGTGCGGGCTCTCGTGAAGCGGATCCTGAAGAGCGCCAGCGAACGGAGCGCCCGGGCC
  GTCAAGACCTTCATGGTCGGGGAGCAGGGGAAGAGCGCCATCGTGATTAGCGGCGTGGGCCTGTTCAGCA
  TTGACTTCGAAGGCGTCGAAGAGGCTGAGCGGATCACCGATATGACCCCCGAGATCGAGTTCGATGAGGA
  CGATGAGGAGGAGGAAGACATCGACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102285 representing YP_145803
  Red=Cloning sites Green=Tags
  
  MADKGMTYSFDVRDNTLVVRRSTATKSGIKISYREDRGTSLLQKAFAGTEDEFWVELDQDVYVDKKIREF
  LVEEKMKDMSTRVSGAVAAAIERSVEFDNFSKEAAANIEMAGVDDEEAGGSGLVDNRRKNKGVSNMAYNL
  SLFIGMVFPALTTFFSAILSEGEMSIWQNGQAIMRILALADEDGKRQTRTGGQRVDMADVTKLNVVTANG
  KVKQVEVNLNDLKAAFRQSRPKRSDYRKGQGSKATESSISNQCMALIMKSVLSADQLFAPGVKMMRTNGF
  NASYTTLAEGANIPSKYLRHMRNCGGVALDLMGMKRIKNSPEGAKSKIFSIIQKKVRGRCRTEEQRLLTS
  ALKISDGENKFQRIMDTLCTSFLIDPPRTTKCFIPPISSLMMYIQEGNSVLAMDFMKNGEDACKICREAK
  LKVGVNSTFTMSVARTCVAVSMVATAFCSADIIENAVPGSERYRSNIKANTTKPKKDSTYTIQGLRLSNV
  RYEARPETSQSNTDRSWQVNVTDSFGGLAVFNQGAIREMLGDGTSETTSVNVRALVKRILKSASERSARA
  VKTFMVGEQGKSAIVISGVGLFSIDFEGVEEAERITDMTPEIEFDEDDEEEEDIDI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006502
• ORF Size: 1848 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_006502.1, YP_145803
• RefSeq ORF: 1848 bp
• Locus ID: 3170816
• MW: 68.0 kDa