P (NC_003461) Virus Tagged ORF Clone

CAT#: VC102044

Myc-DDK-tagged ORF clone of viral ORF for phosphoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604435

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P Protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102044 represents NCBI reference of NP_604435 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGGACGCCTTCTTTTTTGAGCGGGACCCAGAGGCGGAAGGCGAGGCCCCAAGAAAACAGGAGT
  CCTTGTCCGACGTTATCGGTCTCCTTGATGTGGTGCTGTCTTACAAGCCAACTGAAATTGGCGAAGACCG
  GAGTTGGTTGCACGGAATTATCGATAACCCCAAGGAGAATAAACCTAGCTGCAAGGCAGATGATAACAAT
  AAGGACCGAGCTATTTCAACCTCAACGCAGGATCACAGATCTAGTGAGGGTAGCGGGATCTCTCGGAGAA
  CTTCAGAATCTAAAACGGAGACCCACGCCAGGATCTTGGACCAGCAAGGAATTCACAGGGCCTCTCGACG
  GGGAACCAGCCCGAACCCCCTCCCTGAAAACATGGGGAACGAGCGCAACACACGCATCGATGAGGACTCC
  CCAAATGAGCGGCGACACCAGCGATCTGTGCTGACCGATGAAGACCGAAAAATGGCCGAAAACAGCAACA
  AGAGAGAGGAAGACCAGGTTGAGGGATTTCCTGAAGAAGTGCGGCGGAGTACACCTCTGAGCGATGATGG
  CGAGGGGCGGACGAATAATAACGGACGGAGCATGGAGACTAGTAGCACTCATAGCACCCGAATTACAGAT
  GTGATCACTAATCCTTCCCCTGAGCTCGAAGACGCTGTTCTCCAGCGAAATAAGAGGCGACCGACGACCA
  TCAAGAGGAACCAGACACGCTCTGAACGCACCCAATCATCTGAATTGCACAAGTCCACAAGTGAGAATTC
  TAGCAACCTCGAGGATCATAACACCAAAACTTCTCCAAAGGTGCCCCCCAGTAAAAACGAGGAATCAGCC
  GCTACACCTAAAAACAACCACAACCACCGGAAGACCCGCTATACAACAAACAACGCAAACAACAACACCA
  AGTCCCCACCTACCCCCGAGCACGATGCCACCGCAAATGAAGAAGAGACCTCAAACACATCTGTGGACGA
  AATGGCCAAACTTCTTGTGTCCCTGGGAGTCATGAAGAGCCAGCACGAGTTCGAGCTTAGTAGGAGCGCA
  TCACATGTGTTCGCTAAGAGGATGCTGAAATCTGCCAATTACAAAGAAATGACATTCAATCTGTGCGGAA
  TGCTTATCTCAGTAGAGAAGTCCCTGGAAAATAAAGTAGAAGAGAATAGGACACTGCTTAAGCAGATCCA
  GGAGGAAATCAACAGTAGCCGCGATCTGCATAAAAGATTTTCCGAATATCAAAAAGAGCAAAACAGCCTC
  ATGATGGCAAATCTTAGTACACTGCACATCATCACGGACAGGGGCGGAAAGACCGGCAACCCAAGTGACA
  CCACCCGCTCTCCATCAGTCTTCACTAAAGGCAAGGACAATAAAGTAAAGAAAACTAGGTTCGATCCCAG
  TATGGAAGCCCTCGGAGGCCAGGAGTTCAAACCAGACCTGATCAGAGAAGACGAGCTTAGGGATGATATC
  AAGAATCCCGTGCTGGAGGAGAATAATAACGAGCCACAGGCTTCCAATGCCTCCCGACTGATTCCTTCAA
  CAGAGAAGCACACTCTCCATTCTCTGAAACTTGTTATCGAAAACTCACCACTTAGCCGAGTAGAGAAAAA
  AGCATATATTAAGTCCCTGTACAAATGCAGAACGAATCAGGAGGTGAAGAACGTGATGGAGCTTTTTGAG
  GAGGATATAGACTCTCTGACAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102044 representing NP_604435
  Red=Cloning sites Green=Tags
  
  MDQDAFFFERDPEAEGEAPRKQESLSDVIGLLDVVLSYKPTEIGEDRSWLHGIIDNPKENKPSCKADDNN
  KDRAISTSTQDHRSSEGSGISRRTSESKTETHARILDQQGIHRASRRGTSPNPLPENMGNERNTRIDEDS
  PNERRHQRSVLTDEDRKMAENSNKREEDQVEGFPEEVRRSTPLSDDGEGRTNNNGRSMETSSTHSTRITD
  VITNPSPELEDAVLQRNKRRPTTIKRNQTRSERTQSSELHKSTSENSSNLEDHNTKTSPKVPPSKNEESA
  ATPKNNHNHRKTRYTTNNANNNTKSPPTPEHDATANEEETSNTSVDEMAKLLVSLGVMKSQHEFELSRSA
  SHVFAKRMLKSANYKEMTFNLCGMLISVEKSLENKVEENRTLLKQIQEEINSSRDLHKRFSEYQKEQNSL
  MMANLSTLHIITDRGGKTGNPSDTTRSPSVFTKGKDNKVKKTRFDPSMEALGGQEFKPDLIREDELRDDI
  KNPVLEENNNEPQASNASRLIPSTEKHTLHSLKLVIENSPLSRVEKKAYIKSLYKCRTNQEVKNVMELFE
  EDIDSLTN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_003461.1, NP_604435
• RefSeq ORF: 1704 bp
• Locus ID: 935260
• MW: 64.5 kDa