L2 (NC_001596) Virus Tagged ORF Clone

CAT#: VC102019

Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041865

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102019 represents NCBI reference of NP_041865 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCGAGCGAAGCGGACCAAACGGGCCTCCGTCACAGACATCTACAGAGGATGTAAGGCTGCCGGCA
  CCTGCCCCCCCGACGTCATAAATAAGGTCGAGCATACAACCATAGCCGACAAAATTCTGCAGTATGGATC
  AGCCGGCGTGTTTTTCGGTGGGCTCGGCATCTCTACAGGCAGAGGAACGGGAGGTGCGACTGGTTACGTT
  CCCCTTGGCGAGGGACCAGGCGTCCGAGTGGGGGGCACTCCTACCATTGTGCGACCGGGCGTTATCCCCG
  AAATAATTGGCCCCACTGATTTGATCCCTCTGGATACCGTTCGGCCAATCGATCCTACTGCCCCATCAAT
  TGTGACCGGCACTGATTCTACCGTAGACCTGCTGCCAGGAGAGATTGAGTCAATAGCCGAAATACATCCT
  GTACCCGTGGACAACGCCGTGGTCGACACACCCGTGGTGACAGAAGGCCGAAGAGGCAGCAGTGCGATTC
  TGGAGGTAGCAGACCCTTCCCCACCCATGCGGACAAGAGTGGCTCGCACGCAATACCATAATCCTGCGTT
  CCAGATTATCTCTGAGTCCACACCTATGAGTGGTGAGTCAAGCCTCGCTGACCACATAATCGTGTTTGAA
  GGAAGCGGGGGACAGCTGGTTGGCGGACCAAGAGAGTCCTACACCGCAAGTTCCGAGAATATAGAACTGC
  AGGAGTTTCCCAGTCGGTACTCTTTTGAGATTGATGAGGGGACACCACCGCGAACATCCACCCCCGTCCA
  GCGGGCCGTTCAGTCTCTGAGCTCATTGAGAAGGGCTCTGTACAATAGACGCTTGACCGAACAGGTCGCT
  GTAACAGACCCCCTGTTTCTCTCTCGGCCATCAAGGTTGGTGCAGTTCCAATTTGACAACCCGGCTTTTG
  AGGACGAAGTGACACAGATCTTTGAAAGGGATCTCTCCACAGTGGAAGAGCCCCCTGACAGACAGTTCCT
  GGACGTGCAACGACTGAGCCGCCCGTTGTACACAGAAACACCCCAAGGTTATGTGCGCGTGAGCCGGCTG
  GGCAGGAGGGCAACAATACGCACCCGGTCCGGAGCTCAGGTGGGCGCCCAAGTGCACTTTTATAGAGATC
  TGTCCACAATTAATACAGAGGAACCCATCGAGATGCAGCTGCTGGGTGAACATTCCGGTGATAGCACTAT
  CGTGCAGGGTCCAGTGGAGAGTTCTATAGTCGATGTTAATATCGATGAACCCGATGGTTTGGAGGTGGGC
  AGGCAAGAGACTCCCTCCGTGGAAGACGTAGATTTTAATTCCGAGGACCTCCTGTTGGATGAGGGAGTAG
  AGGACTTCAGCGGGAGCCAGTTGGTAGTGGGGACCCGCAGGTCTACTAATACTCTCACAGTCCCTCGCTT
  CGAGACCCCACGGGACACCAGCTTTTATATTCAGGATATCCAAGGGTACACTGTCTCATACCCAGAATCC
  CGCCAGACCACAGATATCATCTTCCCTCATCCGGACACACCAACCGTCGTGATTCATATCAATGATACGA
  GCGGTGACTATTATCTGCATCCTTCCCTGCAGAGAAAGAAGAGAAAAAGAAAATACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102019 representing NP_041865
  Red=Cloning sites Green=Tags
  
  MVRAKRTKRASVTDIYRGCKAAGTCPPDVINKVEHTTIADKILQYGSAGVFFGGLGISTGRGTGGATGYV
  PLGEGPGVRVGGTPTIVRPGVIPEIIGPTDLIPLDTVRPIDPTAPSIVTGTDSTVDLLPGEIESIAEIHP
  VPVDNAVVDTPVVTEGRRGSSAILEVADPSPPMRTRVARTQYHNPAFQIISESTPMSGESSLADHIIVFE
  GSGGQLVGGPRESYTASSENIELQEFPSRYSFEIDEGTPPRTSTPVQRAVQSLSSLRRALYNRRLTEQVA
  VTDPLFLSRPSRLVQFQFDNPAFEDEVTQIFERDLSTVEEPPDRQFLDVQRLSRPLYTETPQGYVRVSRL
  GRRATIRTRSGAQVGAQVHFYRDLSTINTEEPIEMQLLGEHSGDSTIVQGPVESSIVDVNIDEPDGLEVG
  RQETPSVEDVDFNSEDLLLDEGVEDFSGSQLVVGTRRSTNTLTVPRFETPRDTSFYIQDIQGYTVSYPES
  RQTTDIIFPHPDTPTVVIHINDTSGDYYLHPSLQRKKRKRKYL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001596
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001596.1, NP_041865
• RefSeq ORF: 1599 bp
• Locus ID: 1489483
• MW: 58.4 kDa