L2 (NC_001593) Virus Tagged ORF Clone

CAT#: VC101974

Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 53], codon optimized for human cell expression, NP_041847

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101974 represents NCBI reference of NP_041847 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCCATCGCGCAAGACGCAGAAAGAGAGCCTCTGCCACGCAGCTGTACCAGACGTGTAAGCAAA
  GTGGGACCTGCCCCGAGGATGTCATAAATAAGATCGAACATAAAACCTGGGCCGACAAAATCCTGCAGTG
  GGGGTCCCTTTTCACATTCTTCGGTGGACTCGGGATTGGAACCGGGAGTGGCACCGGCGGTAGAACTGGA
  TATATCCCACTCGGGACTAGACCCAGTACCGTGGTTGACGTGACACCCGCTCGGCCCCCTATCGTGGTGG
  AGTCAGTGGGACCGACCGACCCATCCATTGTTACGCTGGTCGAAGAAAGTTCTGTCATCGAGTCAGGAGC
  CTCCTTCCCAAATTTCACAGGGACCGCCGGTTTTGAGGTGACCTCCTCTTCTACTACGACACCAGCCGTG
  CTCGACATCACACCTACATCCACCAGCGTCCACGTGTCATCCACAACATACACGAACCCTACCTTTGTAG
  ATCCCCCTGTGATTGAAGTCCCCCAGACCGGTGAAGTGAGTGGTAATATCCTGATCTCAACCCCTACGTC
  AGGAGTACACAGCTACGAAGAGATTCCAATGCAGACGTTTGCCGTGCAAGGCACGGGGAACGAGCCAATT
  AGCAGCACCCCAATTCCAGGGCTGCGGAGAATCGCTGCTCCCAGGCTGTATAAGAAGGCTTTCCAGCAAG
  TCAAGGTGACAGACCCAGCGTTCCTCCACAAACCTGAGACACTGATTAACGTGGACAACCCTATCTTCCA
  GAATGCCGACACCACCCTCACTTTCTCCCCCAGCGGCGTGGCACCTGATCCCGATTTTCTGGACATTGTA
  GCCCTGCACCGCCCGGCATTCACAACTAGGCGGGGGGGAGTGCGGTTCAGCAGGCTTGGAACCAAGGCGA
  CTATGAGAACGCGGTCTGGCAAACAAATCGGCGCGAGAGTACATTACTACTATGACGTATCTCCAATCAC
  CCAGACAGAAGAAATCGAAATGCAGCCTCTGCTGTCCACGGACAACACATTCGACGGTCTGTACGATATC
  TATGCCAATATCGACGATGAGGCTCCCGTTAGCAGCCGGTTCTCCATTGCCACGCCAAGCCGCCTCCCAA
  CAAACACTGTGCCACTGTCTTTCTCCGGCTCTACCAGCAACGTTACGATACCTTTCGGGACCTCCTGGGA
  CGTTCCCATCTACTCAGGGCCGGATGTCGTCCTGCCAACCGGCCCACCTACCTGGCCCTACGCGCCACAG
  TCTCCGTTCGATACCACCCACGATGTGGTGATTCAGGGGAGCACTTTCGCGTTGTGGCCAGTGTACTTTC
  TCAAAAGAAGGCGAAGGAAAAGGATCCCTTACTTCCTCGCAGACGGTGGCGTGGCCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101974 representing NP_041847
  Red=Cloning sites Green=Tags
  
  MVAHRARRRKRASATQLYQTCKQSGTCPEDVINKIEHKTWADKILQWGSLFTFFGGLGIGTGSGTGGRTG
  YIPLGTRPSTVVDVTPARPPIVVESVGPTDPSIVTLVEESSVIESGASFPNFTGTAGFEVTSSSTTTPAV
  LDITPTSTSVHVSSTTYTNPTFVDPPVIEVPQTGEVSGNILISTPTSGVHSYEEIPMQTFAVQGTGNEPI
  SSTPIPGLRRIAAPRLYKKAFQQVKVTDPAFLHKPETLINVDNPIFQNADTTLTFSPSGVAPDPDFLDIV
  ALHRPAFTTRRGGVRFSRLGTKATMRTRSGKQIGARVHYYYDVSPITQTEEIEMQPLLSTDNTFDGLYDI
  YANIDDEAPVSSRFSIATPSRLPTNTVPLSFSGSTSNVTIPFGTSWDVPIYSGPDVVLPTGPPTWPYAPQ
  SPFDTTHDVVIQGSTFALWPVYFLKRRRRKRIPYFLADGGVAA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001593
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001593.1, NP_041847
• RefSeq ORF: 1389 bp
• Locus ID: 1489467
• MW: 50.1 kDa