L1 (NC_001691) Virus Tagged ORF Clone

CAT#: VC101970

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043429

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101970 represents NCBI reference of NP_043429 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACACTGGTCCTCCACGAGCGGCAAGTTGTACCTCCCACCCAGCACCCCAGTGGCTCGAGTCTTGT
  CAACAGACGAATACGTGAAGGAGACCGATGTCTACTTCCATGCCCGCAGCGAAAGGCTCCTGATCGTTGG
  CCACCCATATTACGATATTGAGGATGGAGGTGATATTAAAGTTCCAAAAGTGTCTGCCAACCAGTATCGC
  GTGTTCCGGTGTGAGCTTCCTGATCCTAACAAATTTGCTTTGATTGACACAACATTGTACAATAGCGACA
  CGGAAAGACTGGTGTGGAAATTGGTGGGGATCGAGGTCGGAAGGGGAGGACCGCTGGGCGTAGGGTCAAC
  GGGGCACCCCCTGTTCAACAAAGTGGGCGATACTGAGAACCCCAGCTTCTACCTGGGACCACAGGAAAAG
  GACGAAAGGCAAAATCTGTCAATCGACCCAAAGCAAACTCAACTGCTCATAGTAGGATGTAAGCCCGCCG
  TGGGAGAGTATTGGGATCTCGCAGAGCCGTGCGACAAGAACTCCCTTAACAACGGAAAATGCCCCCCCAT
  TCAACTCGTCAATTCCTACATCCAGGACGGGGATATGGGAGACATTGGTTTTGGGAATGCCAACTTCCCC
  AAACTGCAGCAGGATCGGGCAGGGGTGCCTCTTGATATCGTTGACTCAATCAGTTTGTGGCCGGACCTTT
  TGAAGATGACAAAGGATGTCTATGGCGACCATGTTTTCTTCTACGCTAAACAGGAACAGTTGTACGCGAG
  GCACCTCTTCACCCATGCGGGGCCGATCGGCGAGCCTATCCCCAACGTTTCTGGGGTTTACAATTACGCC
  GTCAACCCGAACCAGCCGGAGCAGAATCGCCGGACCAATATAGGCTCATACCTGTATTTCACCACCCCGA
  GCGGGAGCCTGAACACATCTTCCAGTCAGCTTTTCAACCGGCCCTATTGGATCCGGAGAGCTCAAGGAAC
  AAACAACTGTATCTGCTGGGGCAACGAGGTCTTCGTGACGGTTTTCGACAACACCAGGAATATTAACTTC
  AATATTTCCGTTAAAAAGGATGTCAATCCACTCGACCCTCTCAACGTTGCTTCTAGCTACATGTACTCAA
  AGGACGACTTTAATCAGTATTCTCGCCATACAGAAGAATATGAGCTTGAGTTCATTTTTCAGCTGTGTAA
  AGTGGGCTTGGATGCAGACATCTTGGCACACCTCAATGTGATGGATCCGCGGATCTTGGAAAATTGGCAG
  TTGGCGTACGTGCCCCCAGCTCCCTCTGGTATCGGGGATACCTACAGGTACCTGAAGTCTGACGCAACCA
  AGTGCCCTGCCAAAGACAGCAGCGCAGAGGTCGTGGACCCATATAAGGAGTATACCTTTTGGAATGTCAA
  TCTGACAGAGAAATTCTCATCCGAACTGGACCAGTACGCCCTGGGGCGAAAATTTCTCTTCCAGACCGGT
  TTGCTCAAGCGCCGAGTCAGAACCGACTACACTGTCGCTACCGTCAGCAAACCAAATAAGCGGAAAAGAA
  CCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101970 representing NP_043429
  Red=Cloning sites Green=Tags
  
  MAHWSSTSGKLYLPPSTPVARVLSTDEYVKETDVYFHARSERLLIVGHPYYDIEDGGDIKVPKVSANQYR
  VFRCELPDPNKFALIDTTLYNSDTERLVWKLVGIEVGRGGPLGVGSTGHPLFNKVGDTENPSFYLGPQEK
  DERQNLSIDPKQTQLLIVGCKPAVGEYWDLAEPCDKNSLNNGKCPPIQLVNSYIQDGDMGDIGFGNANFP
  KLQQDRAGVPLDIVDSISLWPDLLKMTKDVYGDHVFFYAKQEQLYARHLFTHAGPIGEPIPNVSGVYNYA
  VNPNQPEQNRRTNIGSYLYFTTPSGSLNTSSSQLFNRPYWIRRAQGTNNCICWGNEVFVTVFDNTRNINF
  NISVKKDVNPLDPLNVASSYMYSKDDFNQYSRHTEEYELEFIFQLCKVGLDADILAHLNVMDPRILENWQ
  LAYVPPAPSGIGDTYRYLKSDATKCPAKDSSAEVVDPYKEYTFWNVNLTEKFSSELDQYALGRKFLFQTG
  LLKRRVRTDYTVATVSKPNKRKRTR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001691
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001691.1, NP_043429
• RefSeq ORF: 1545 bp
• Locus ID: 1403635
• MW: 58.4 kDa