hypothetical protein (NC_001457) Virus Tagged ORF Clone

CAT#: VC101930

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp6 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040894

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101930 represents NCBI reference of NP_040894 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATCCTTGAGCAGACGAAAGAGAGATTCTGTCCCCAACCTGTACGCTAAATGCCAGCTTAGCGGCA
  ACTGCCTGCCCGACGTGAAGAATAAGGTGGAGGCCGACACCCTCGCGGATAGGCTGCTGAGATGGCTGGG
  CTCTGTGATCTATCTGGGTGGCCTCGGAATCGGAACCGGCAGGGGAAGCGGGGGAAGCACCGGATATAAT
  CCGATCGGAGCCCCTTCCAGAGTGACACCAAGCGGTACACTCGTGAGACCTACCGTGCCCGTGGAGAGCC
  TGGGACCAAGCGAGATCATCCCTATCGACGCCATTGATCCAACAACTAGTTCAGTGGTGCCACTGGAGGA
  TTTGACTATTCCGGATGTTACAGTCGACTCTGGCGATACCCGAGGCATCGGAGAAACCACACTGCAGCCC
  GCACAGGTTGATATCAGCACGAGCCATGACCCAATCTCAGATGTGACGGGGGCAAGTTCACACCCAACAA
  TTATAAGCGGCGAGGATAACGCGATTGCTGTGCTGGATGTAAGTCCTATTGAACCGCCTACTAAGAGAAT
  TGCACTTGCCACAAGAGGGGCTAGCGCGACTCCCCATGTGTCAGTTATTTCAGGAACTACAGAATTCGGA
  CAAAGCAGCGACCTGAATGTATTCGTTAACGCTACATTTAGCGGGGACTCTATTGGGTACACTGAGGAGA
  TTCCGCTGGAACCCCTTAACCCATTCCAGGAATTCGAAATTGAGAGCCCTCCCAAAACGTCAACTCCACG
  CGATGTTCTGAATCGCGCTATTGGACGCGCAAGGGACCTGTATAATCGCAGAGTCCAGCAAATCCCCACA
  CGGAACCCTGCTCTCTTGACCCAGCCTTCACGAGCCATTGTATTTGGATTCGAAAATCCCGCATTTGACG
  CAGACATTACACAAACCTTCGAGCGAGACCTCGAGCAGGTAGCCGCCGCACCGGACGCTGATTTTGCTGA
  TATTGTGACAATAGGACGCCCGCGCTTCTCAGAGACAGACGCCGGTCAAATTAGGGTCTCCAGACTCGGA
  AGGAGAGGTACAATCAAAACACGCAGTGGGGTTCAGATCGGCCAGGCCGTACACTTCTACTACGACTTGT
  CTACCATCGATACTGCAGACGCGATCGAGCTGTCCACACTGGGGCAACACAGCGGTGAACAAAGCATCGT
  GGACGCCATGATAGAATCTTCACTGATTGATCCATTTGAGATGCCCGACCCCACCTTCACAGAAGAACAG
  CAGTTGCTGGATCCACTCACAGAGGATTTCTCACAAAGCCACCTGGTCCTCACATCAAGCCGCCGGGGAA
  CTTCCTTCACAATACCAACAATACCGCCAGGGCTCGGTCTTCGAATTTATGTGGATGATGTAGGCAGTGA
  TCTGTTTGTGAGCTACCCTGAAAGTAGAGTCATACCCGCCGGGGGTCTCCCAACCGAGCCTTTCGTGCCT
  CTTGAGCCCGCCCTGCTCTCAGATATCTTCTCAACCGATTTTGTGTACCGGCCGAGTTTGTATAGGAAGA
  AGCGGAAGCGCCTCGAGATGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101930 representing NP_040894
  Red=Cloning sites Green=Tags
  
  MQSLSRRKRDSVPNLYAKCQLSGNCLPDVKNKVEADTLADRLLRWLGSVIYLGGLGIGTGRGSGGSTGYN
  PIGAPSRVTPSGTLVRPTVPVESLGPSEIIPIDAIDPTTSSVVPLEDLTIPDVTVDSGDTRGIGETTLQP
  AQVDISTSHDPISDVTGASSHPTIISGEDNAIAVLDVSPIEPPTKRIALATRGASATPHVSVISGTTEFG
  QSSDLNVFVNATFSGDSIGYTEEIPLEPLNPFQEFEIESPPKTSTPRDVLNRAIGRARDLYNRRVQQIPT
  RNPALLTQPSRAIVFGFENPAFDADITQTFERDLEQVAAAPDADFADIVTIGRPRFSETDAGQIRVSRLG
  RRGTIKTRSGVQIGQAVHFYYDLSTIDTADAIELSTLGQHSGEQSIVDAMIESSLIDPFEMPDPTFTEEQ
  QLLDPLTEDFSQSHLVLTSSRRGTSFTIPTIPPGLGLRIYVDDVGSDLFVSYPESRVIPAGGLPTEPFVP
  LEPALLSDIFSTDFVYRPSLYRKKRKRLEMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001457
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001457.1, NP_040894
• RefSeq ORF: 1563 bp
• Locus ID: 1489451
• MW: 56.3 kDa