E1 (NC_001587) Virus Tagged ORF Clone
CAT#: VC101921
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041809
View other clones from "Virus" (5)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | E1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC101921 represents NCBI reference of NP_041809 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACTCTGGAAACTGGGAGGGGCGGTGTAGCGGATGGTTCAATGTTGAGGCAATCGTTGAACGGA AAACGGGAGACGCAATTCCAGCGGATGAGAATTATGACGGCGACGACACTGAGGACTCAGAGATGGGTGA CTTCATCGACAACGCCCATATCAGCAACATTTACTCTCAGCAGGAAATTGCTCAAGCACTGTACCACAGC CAGCAGGTTAACGCTGATAATGAAGCAATTCGAGTGCTGAAAAGAAAGTTTGCAGGCTCTGCTGGGAGCT CCCCCGATTCCAAGCGACATGAATTGAAGCACAAGCAAAGATCTCCACATATACTGACCATTAGGGACAC AAACACAACCTCTACCCACCTGCTGTGCGAGGAGCAGGATTCCGGATATGGCAACACTGAGGTCGAGACC TATGAAAGGCAGGTGCCCGGACCCGGCGGGTGTTTGCAGTCCACATCCTCATCCAACAACGGAAGTCAAA TGGCCAGTCCAGGTGAGACGAACTCTGGTAGCAGTAGCATATCCAACATGGATATAGACATGGAGTCCAC CCCTATTACTGACATCACCAATATCCTCAAAAGTTCAAACGTGAAAGCTACGCTTCTTGCTAAATTTAAG GAAGTCTACGGCCTGAGCTATATGGAGCTGGTGCGCCCCTATAAGTCAGATAAAACACAGTGTCAGGACT GGGTGTGCGCTGTCTTCGGTGTGGCGCCGAGCCTCGCCGAGAGTCTGAAAAGCTTGCTGACTCAGTATTG CCTGTACATTCACCTCCAGTGTTTGACTTGCAGCTGGGGCATTATCGTGCTGCTGCTGGCCCGGTTCAAG TGCAACAAAAATAGACTGACAGTCCAGAAACTCCTGCACGGGCTCCTGAACGTGACACAAGAGTATATGC TCATCGAACCTCCAAGATTGAGGTCTACCCCCTGCGCTTTGTACTGGTACCGAACCTCACTGTCAAACAT AAGCGAAACAGTAGGGGAGGTACCCGAGTGGATAAAACGGCAGACCGTGGTGCAGCACAGCCTGGAGGAT TGTCAGTTTGATCTCTCCCAGATGGTTCAGTGGGCCTTCGATAACGATATTACTAACGATTGCGAAATCG CCTACAAGTACGCACTCTTGGCTTCAGAGGACTCTAATGCCGCTGCCTTTCTCAAAAGTAATGCCCAGGC CAAGTACGTGAAGGACTGCGGCACAATGTGCCGGCACTACAAGGCCGCTGAACGGAAGCAAATGACCATG TCACAATGGATAACACACAGATGCGACCTCATTGATGACGGAGGCAACTGGAAGCATATCGTGCAATTTC TCCGCTACCAGCAGGTCGAATTCGTGCCATTTCTGATAGCCTTGAAGCAGTTTCTTAAGGGTATCCCCAA GCAGAACTGTATTGTGATCTATGGGCCACCTGACACTGGAAAGTCACACTTCGGCATGAGCCTGATGCAG TTCATGCAGGGGGTCGTCATCTCTTACGTAAACTCTAACAGCCACTTTTGGCTGTCTCCTCTGGCCGACG CTAAAATGGCTCTGCTTGACGACGCCACTCCGGCCTGTTGGACCTACATCGACAGATATTTGAGGAATGC TCTCGACGGAAATCCAATGTGCTTGGATAGGAAACACAAACACCTGTTGCAAATTAAATGCCCACCACTT CTGATCACTAGCAATACAAATCCCAAGGCTGATGACACATGGAAATACTTGCACAGTCGAATGAAGGTAT TCACCTTTTCTAATCCATTTCCATTCGACTCAAACGGCAACCCACTCTACCAACTGACAAACGAAAACTG GAAAGCCTTTTTCACCAAGACTTGGTCAAAACTGGACCTGACAGAGGACGACGATAAAGAGAATGACGGC GACACAGTGCAGACCTTTAAGTGCGTTAGTGGCAGGAATCCCCGGACCGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101921 representing NP_041809
Red=Cloning sites Green=Tags MADSGNWEGRCSGWFNVEAIVERKTGDAIPADENYDGDDTEDSEMGDFIDNAHISNIYSQQEIAQALYHS QQVNADNEAIRVLKRKFAGSAGSSPDSKRHELKHKQRSPHILTIRDTNTTSTHLLCEEQDSGYGNTEVET YERQVPGPGGCLQSTSSSNNGSQMASPGETNSGSSSISNMDIDMESTPITDITNILKSSNVKATLLAKFK EVYGLSYMELVRPYKSDKTQCQDWVCAVFGVAPSLAESLKSLLTQYCLYIHLQCLTCSWGIIVLLLARFK CNKNRLTVQKLLHGLLNVTQEYMLIEPPRLRSTPCALYWYRTSLSNISETVGEVPEWIKRQTVVQHSLED CQFDLSQMVQWAFDNDITNDCEIAYKYALLASEDSNAAAFLKSNAQAKYVKDCGTMCRHYKAAERKQMTM SQWITHRCDLIDDGGNWKHIVQFLRYQQVEFVPFLIALKQFLKGIPKQNCIVIYGPPDTGKSHFGMSLMQ FMQGVVISYVNSNSHFWLSPLADAKMALLDDATPACWTYIDRYLRNALDGNPMCLDRKHKHLLQIKCPPL LITSNTNPKADDTWKYLHSRMKVFTFSNPFPFDSNGNPLYQLTNENWKAFFTKTWSKLDLTEDDDKENDG DTVQTFKCVSGRNPRTV TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001587 |
ORF Size | 1941 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_001587.1, NP_041809 |
RefSeq ORF | 1941 bp |
Locus ID | 1489429 |
MW | 73.3 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC101919 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041807 |
USD 165.00 |
|
VC101920 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041808 |
USD 165.00 |
|
VC101922 | Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041810 |
USD 503.00 |
|
VC101923 | Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041811 |
USD 503.00 |
|
VC101924 | Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041812 |
USD 541.00 |
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