E7 (NC_012213) Virus Tagged ORF Clone
CAT#: VC101845
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for early protein E7 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647034
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Product Images
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Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | E7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC101845 represents NCBI reference of YP_002647034 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAGGAAAAGCACCCACAATCAAAGACGTCGACTTGGAACTCCATTCACTGATTTTGCCCGAAAACC TCCTGTGTGGAGAAGAGGTTACCTTTGAAAAGGAGGAGCAGGAGGAACAGGACAAGTGCCCTTATCGGGT CGTAACGGAGTGTGGAGTGTGCCATAAGGGATTGTGCATGTATGTGCTGGCCACAGATGAGGGCATCCGC GGATTCGAACAACAACTTTGTGGAGACCTCGACATTGTGTGCCCAGCATGCGCCAAGCAAAGGGAACGGA ACGGGGGTCAGAGGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101845 representing YP_002647034
Red=Cloning sites Green=Tags MRGKAPTIKDVDLELHSLILPENLLCGEEVTFEKEEQEEQDKCPYRVVTECGVCHKGLCMYVLATDEGIR GFEQQLCGDLDIVCPACAKQRERNGGQRH TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_012213 |
ORF Size | 297 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_012213.1, YP_002647034 |
RefSeq ORF | 297 bp |
Locus ID | 7559100 |
MW | 11.2 kDa |
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC101846 | Myc-DDK-tagged ORF clone of viral ORF for early protein E1 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647035 |
USD 641.00 |
|
VC101847 | Myc-DDK-tagged ORF clone of viral ORF for early protein E2 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647036 |
USD 503.00 |
|
VC101848 | Myc-DDK-tagged ORF clone of viral ORF for late gene L2 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647037 |
USD 529.00 |
|
VC101849 | Myc-DDK-tagged ORF clone of viral ORF for early protein L1 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647038 |
USD 525.00 |
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