L2 (NC_001356) Virus Tagged ORF Clone

CAT#: VC101765

Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Mupapillomavirus 1], codon optimized for human cell expression, NP_040308

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101765 represents NCBI reference of NP_040308 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCGCCTGCGCCGAAAAAGAGCGGCCCCAAAGGACATTTACCCCTCTTGTAAGATTTCTAATACCT
  GTCCTCCTGATATCCAAAACAAAATTGAACATACTACGATCGCGGATAAGATTTTGCAGTACGGATCTCT
  TGGAGTGTTTCTCGGCGGACTTGGAATTGGCACAGCAAGAGGATCAGGCGGCCGGATCGGGTACACGCCC
  CTGGGTGAGGGGGGGGGAGTCCGGGTCGCTACAAGACCCACCCCTGTGCGGCCAACTATCCCTGTAGAGA
  CGGTAGGACCCAGTGAGATTTTTCCCATCGACGTGGTCGACCCCACCGGCCCCGCCGTGATACCCTTGCA
  GGATCTGGGAAGGGACTTCCCCATCCCTACCGTGCAGGTGATCGCTGAAATTCATCCGATATCAGACATC
  CCCAACATAGTTGCCTCTTCAACTAACGAAGGCGAAAGTGCTATTCTGGACGTCCTGCGAGGGAACGCAA
  CCATCAGAACTGTGTCAAGAACACAGTATAATAATCCCTCATTCACTGTAGCTTCTACCTCAAACATTAG
  CGCAGGAGAAGCTTCCACCTCTGACATAGTGTTTGTGAGCAACGGGTCTGGAGACCGCGTAGTCGGCGAG
  GACATCCCTCTGGTCGAGCTGAACTTGGGCCTTGAGACGGATACCTCTTCCGTAGTCCAGGAAACAGCAT
  TTAGTAGCTCAACCCCTATCGCCGAAAGACCATCCTTTCGACCTTCCAGATTCTACAATAGACGCCTGTA
  CGAGCAAGTGCAGGTCCAGGATCCTCGCTTCGTGGAACAGCCCCAGAGCATGGTGACGTTCGACAATCCA
  GCATTTGAGCCCGAACTTGACGAGGTTTCAATCATCTTTCAGCGCGACCTCGACGCCCTCGCCCAGACCC
  CTGTCCCGGAATTTCGGGACGTCGTATATCTGTCAAAACCTACTTTTAGCCGCGAGCCTGGAGGAAGACT
  CAGGGTATCCCGCCTGGGAAAATCATCCACGATTCGAACGAGGCTGGGCACCGCAATCGGGGCTCGGACT
  CACTTCTTCTATGACCTTAGCAGTATTGCCCCTGAAGACAGTATCGAACTCCTGCCGCTGGGAGAGCACA
  GCCAAACAACAGTAATCTCCTCCAACCTTGGCGACACCGCGTTTATCCAAGGAGAGACTGCCGAAGACGA
  CCTGGAAGTTATAAGTTTGGAAACTCCTCAGCTCTACTCAGAGGAGGAACTGCTGGATACAAATGAGAGC
  GTGGGCGAAAACTTGCAGCTCACCATTACCAACAGCGAGGGCGAGGTGTCAATTCTCGACCTGACCCAGT
  CCAGGGTGCGCCCACCTTTCGGAACCGAGGATACCTCACTCCACGTGTACTACCCAAATAGTAGTAAGGG
  AACTCCAATTATCAATCCTGAAGAATCATTCACACCACTGGTCATCATTGCCCTGAACAATAGCACCGGC
  GATTTTGAACTTCATCCATCACTGAGGAAAAGGAGGAAGAGAGCTTACGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101765 representing NP_040308
  Red=Cloning sites Green=Tags
  
  MYRLRRKRAAPKDIYPSCKISNTCPPDIQNKIEHTTIADKILQYGSLGVFLGGLGIGTARGSGGRIGYTP
  LGEGGGVRVATRPTPVRPTIPVETVGPSEIFPIDVVDPTGPAVIPLQDLGRDFPIPTVQVIAEIHPISDI
  PNIVASSTNEGESAILDVLRGNATIRTVSRTQYNNPSFTVASTSNISAGEASTSDIVFVSNGSGDRVVGE
  DIPLVELNLGLETDTSSVVQETAFSSSTPIAERPSFRPSRFYNRRLYEQVQVQDPRFVEQPQSMVTFDNP
  AFEPELDEVSIIFQRDLDALAQTPVPEFRDVVYLSKPTFSREPGGRLRVSRLGKSSTIRTRLGTAIGART
  HFFYDLSSIAPEDSIELLPLGEHSQTTVISSNLGDTAFIQGETAEDDLEVISLETPQLYSEEELLDTNES
  VGENLQLTITNSEGEVSILDLTQSRVRPPFGTEDTSLHVYYPNSSKGTPIINPEESFTPLVIIALNNSTG
  DFELHPSLRKRRKRAYV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001356
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001356.1, NP_040308
• RefSeq ORF: 1521 bp
• Locus ID: 1489172
• MW: 55.3 kDa