U77 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101516

Myc-DDK-tagged ORF clone of viral ORF for Helicase/primase complex [Human herpesvirus 6], codon optimized for human cell expression, NP_050256

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101516 represents NCBI reference of NP_050256 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTATTAGTAGCCTTTTCGGAGGTCGGTACGACAATAAGTTCCTCTTGAACATGTCATCTGCCCCAA
  AGATAGAACTGATCGTCGACAAGGTGGCATCCCTGTCCGAAAGACGGCTCGAAGGCCGGCTCCCAGAGGA
  CTGGTTTCGCCACATCATGGACCCAGAGACAGAGTTCAATGGTGAATTCGCCGACGCCCTGTGCATTGGA
  ATTGACGAATTCGCGCAACCCCTGCCCTTCCTGCCTTTCAAAGCTCTGCTGGTCACTGGGACCGCAGGCG
  CGGGGAAAACCAACTCTATACAGACTCTGGCCGCAAATTTGGACTGCATAGTGACAGCTACCACCTCAAT
  TGCGGCCCAGAACTTGTCTGTGGTGCTGAATAGATCCAAGTCCGCCCAGGTGAAAACCATCTTCAAGACG
  TTCGGGTTCAACTCAAGCCACGTGTCTATGTCTGAAAGGCAATCCTACATAGCCAATGACGAGAGGTCCA
  TCCAAATTCAACAGAAGCAGGACCTGAGTATATATTGGAATGTTATAAGTGACATCGCCGAGCGAGCCCT
  TGGTGCCGTGGCATGCAAGACCAAAGAACTGCCAGATTTGTGCGAGTCAAGCGTGATAGTTATAGATGAA
  GCAGGCGTGATACTCAGACACATTCTTCATACCGTCGTATTCTTTTATTGGTTTTACAATGCATTGTACA
  AGACTCCTTTGTACGAGAACGGCATTGTTCCTTGCATTGTGTGTGTAGGGAGTCCGACTCAGTCCAATGC
  ACTCGTGACTTCTTTCAACCCACTTACACAGAATAAGGATGTCAAGCGGGGCATTGACGTCCTGAGTGCA
  CTGATCTGTGATGACGTGCTGTCTAAGTATTGCGAAGTTGATAATAACTGGATAATCTTTGTCAATAACA
  AGAGATGCGCAGATCACGCCTTCGGGGACTTTCTGAAGCACATAGAATTCGGACTGCCACTTAAGCCCGA
  ACTGATCGAATATGTTGATCAATTTGTGAAGCCCGCATCATACATTCGCAACCCAATGAACGAGATTGAA
  ACAACCCGGCTGTTTCTGTCCCACAACGAAGTTAAAAACTACTTTAGGAGCCTCCACGAACAGGTCGAAG
  TGACTAATCGGAATAACCTGTTTGTCTTTCCAGTATACTTTCTGATCAAGAATAAGACATTCGAAGACTA
  TAAATCAGAAATCGGTAACTTTAGCCTGGAAATTGAGCCATGGTTCAAGAGCAACATCCACCGGCTTAAC
  ACTTACTCTCAATTCGCAGACCAGGATCTGTCTAAGACAGTTCAACTTGAAGAGATCGTGCTTGAGGATG
  GGTCCGTGGAAGAAACCCTTATCACATGTCATCTCAAGCACATTCGGAATAGTTCCATTGGAGTGACATC
  TAAGATAAAAGCCTCAACCGTCGGGTTTAGCGGAACTTATGAAAAGTTCGTGGAGCTGCTGCAAAGCGAT
  CTCTTTATTGAGAAGACCTCATGCGAGCAAACCATTCACGCCTACAGTTTTCTGAGCGGCCTCATGTTTG
  GCGGCATGTACAGCTTTTGTTGTAGCGAGTTCACTACGCCGGAAGTGCTGATGGAAATAAAAAATATCAA
  AATGCCATCCATCGAATTTCTCGAGTCCGAGATGTCCAGGATGAGTAGGGACGTACAAACAGTGGAGACA
  GATGAGCGGTATGACTTTGGCCTTGTGGATGATGGCCTGTCTGATATGGATCTCCTGGAGATCGATCCAT
  GTGGCGATCCGTTTTTTACTCGGTACTCTAAGTTGCCGCTCACTAACTCCCTTAGCTTCGAGGAAATTAG
  CCTGCTGTACACCACCTTCAAAGATATCTTCATCAGTCGCTTTGCTATCCTGCAAAAACATACTAAGGGG
  AAATTTGGAAAAACCCTTCTGGTGACCTACAATCGCAATAACGTTTCCCGCAAACAGTGCGGCGAAATCT
  ATTCACATCTGAAGAGTTTTTACGGCATGTTGACATATGCCATCCCCGCGAATAACTATACCCTTGAGGG
  CTATACAAACGATAACGTGGTTCACCTGGGAACGGACAAACAGCTCCCGCAGATTCTGTACAAGAAAGGT
  CTGCCTCGCTTGGTTATCAAGGACGAAATGGGTTTTATTTCAGTTCTGGACAACAACGTGTCCAAATTTG
  TAGACGTGGTGAATGGGCAGTCTTTCCACCTGTGCACGACAGTGGACTACGCCACCGTGTCTAAAGTGAG
  TATGACCATCACTAAAAGCCAGGGCCTGAGTATCCAGAAAGTAGCTATTGACTTTGGTTCTGATCCAAAG
  AATCTGAAGCTCTCCTCTATCTATGTCGGTATGTCAAGAGTCACAGACCCAAATAATCTGATTATGAACG
  TGAACCCTCTCAGATTGAACTACGAGAACGACAATTTTATCGCCCCACATATTGTGAAAGCTCTCAAAAA
  CGAAAATACTATGCTCATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101516 representing NP_050256
  Red=Cloning sites Green=Tags
  
  MSISSLFGGRYDNKFLLNMSSAPKIELIVDKVASLSERRLEGRLPEDWFRHIMDPETEFNGEFADALCIG
  IDEFAQPLPFLPFKALLVTGTAGAGKTNSIQTLAANLDCIVTATTSIAAQNLSVVLNRSKSAQVKTIFKT
  FGFNSSHVSMSERQSYIANDERSIQIQQKQDLSIYWNVISDIAERALGAVACKTKELPDLCESSVIVIDE
  AGVILRHILHTVVFFYWFYNALYKTPLYENGIVPCIVCVGSPTQSNALVTSFNPLTQNKDVKRGIDVLSA
  LICDDVLSKYCEVDNNWIIFVNNKRCADHAFGDFLKHIEFGLPLKPELIEYVDQFVKPASYIRNPMNEIE
  TTRLFLSHNEVKNYFRSLHEQVEVTNRNNLFVFPVYFLIKNKTFEDYKSEIGNFSLEIEPWFKSNIHRLN
  TYSQFADQDLSKTVQLEEIVLEDGSVEETLITCHLKHIRNSSIGVTSKIKASTVGFSGTYEKFVELLQSD
  LFIEKTSCEQTIHAYSFLSGLMFGGMYSFCCSEFTTPEVLMEIKNIKMPSIEFLESEMSRMSRDVQTVET
  DERYDFGLVDDGLSDMDLLEIDPCGDPFFTRYSKLPLTNSLSFEEISLLYTTFKDIFISRFAILQKHTKG
  KFGKTLLVTYNRNNVSRKQCGEIYSHLKSFYGMLTYAIPANNYTLEGYTNDNVVHLGTDKQLPQILYKKG
  LPRLVIKDEMGFISVLDNNVSKFVDVVNGQSFHLCTTVDYATVSKVSMTITKSQGLSIQKVAIDFGSDPK
  NLKLSSIYVGMSRVTDPNNLIMNVNPLRLNYENDNFIAPHIVKALKNENTMLIF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2472 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_000898.1, NP_050256
• RefSeq ORF: 2472 bp
• Locus ID: 1497077
• MW: 93.4 kDa