U30 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101471

Myc-DDK-tagged ORF clone of viral ORF for capsid assembly protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050211

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101471 represents NCBI reference of NP_050211 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCACGACAACAGGCAGCTGTTGCCCCAGGAGACCAAGCTGGTCACACCCAACAAAGATACTCTGT
  CTAGTCGGTACCTGCACGTTCTGATAATTGATAACACCCTGAGCACTATAGAATTCGTGTATATGGTGGT
  GAAAGCCGTGCTGACGCAGGCTGACACCCTTAAAGCCTTCGAGCAGAGGAAGAACAGGCCAACAAATAGG
  ATCCTGGGGCTCAGCACCAGCGTTTCAAAGAGATACATCAATATTCCGAAATTTAGCAGCTCAGGCGCAA
  ATAACTCACAGGGCGTTAAAACGCTCGCCGCCATACGCAAGTTTTCCTTGCCCGACAAGAATTGTTTTCG
  AAACTTCCTGAGCTTCTCAACCACTCTTTTTAAGGTTCCGGCCTCCATCGACATTTATTTTCTTTTCTTC
  ACAGCCAGTACCGTTTCTACTATGGCCGCACGGGCTCTGGACCTTGAGTTCATCCTCTCTAGCTTGAAAA
  ATTCTACAAGTCCCGAGTCCCTGCTGGCAGCCACAGCTAAAATAGAGATTCTGTCTCTGGCTGCCGATTC
  AGTAACCCATAATCGAGTGATCGCATTTATTAACAGACTCCCACCTAAGAAATATCACTTTGATCTCATC
  CGAGAATATACAGTTTTCTACTTCCTGAATAGTACAACCCTCACCTCCGAGAATAAGCTGCTCAGCGCCG
  AGCTCCTCCATGAGGAACTCTCCCAGATCCGGTCTTCTTCCTCACCTGGGACCGAGCTCGAGAACCTGAA
  TAACGCTGAGGTGTTGTATGTTTTCGATCGGATACTGAATAGTATAAAAATGCTGAAGAACGAACTCTCT
  AGCCCTATAGGTAAGCTGCGGGTGCAGCCCGCGGCAAACGATCCCGGGACTGATAAGACTATCAAGTATT
  CCAAAATCCAAAGTATTATCCAAAAAGTGCATAGTTTTACAAGGGAGAACAGCTTGCTCGCGAACTGCAG
  GGCCGTGGTGGAACTGATTGATGATCTGTACCGGAAACTTTATAGCTGGTTTCTGCACATTCTGACATTT
  GAAGATATTCAATTTCCGGGGGATACCTTTCTCGACCGCCTGCTGAAGATGGACTACTGTTTCACATATT
  ATCCTAGCTCTAATAGGCACCTGATAGACCTCTTTGAAAAAACTTTGGACAATCAGACTTCCACCGACAT
  CGATAAGTTCTTCGACACTAGCGGAAATAGCCCGGAGCTGCTCTACCAGAAAACCTTTAGCCTGAAGATT
  TTTAGCAAAAATCTCACGGCACAGGACAATGGCCTGTACATCTATCCGCTGTTGAAAACCGATCTGAGCA
  TTCTGGATTTTCTCGGGACCGAAAACATCCTTTTCCATAGAGGACTGATATATCACATCCTGCATCAGAA
  AACGATCCCGCAAGAACGGGAGAACGATCTGAATAAGATCAATCAATTCTTCGCAACGGTAATCCAACAA
  GTCATTGAGACTCGCAGTTCTTGTCTGCCTGCAAGCCTGTCCAGGCTGCTGGACACTATCTTTCACTTCA
  ATCGGATTGGACTCAACATCGAAACATGCAGGATTTACGTGGAGATACTGTCAAACCATATGGCCACCCC
  CGACACACAGCCAATCATTAACACCTTCACTATAAATCTTATCCACATCGTATTTACCGCGCATGTGTTC
  TTCATTTGTATGGAGAACTTCTCTCCCACCTTCCTGTTTTACAACAGGAAGAAACTGATTCTGGAACAGC
  AGCGCGCCATCTTGATCATTGAACGAAACGAGTATTCAACTCTCTGGAAGCAGATTTCTGATCACATCGA
  CTGCTTGTTCAATATCTCTCTCAGCGAAAGTTTCTTCAAGGAATACACTAAAGGAGGCAACGAAGAACAC
  AAACAGTTCCTGTATAAGAATCTGTTTGAGAAATGGGGTGATGTGTTTTTCCCTTTTACGTATAGTGTGA
  CCACCTCAAAAAATTCTACAGCACATCATATCACGACCCTGGAGCTGCGGGCTATATGCAAGGAGGTTTA
  TCAGTCTGACTCACCTGAAGCGTATGAATCTCTTTTGCCATACTCTACCCATCCCGCATTCAAGACTCTG
  TTTATTAAGATTTATGTCATACCCATGGTTACCTATATTACTAATCTGACATTCGACAAACTCCAATCTG
  ATTATAGGCTCATTACTCTGATCCATGCTTGTAAGCTGCTGTTGCCCTCTCAGCACCTGCTCCTTCACTA
  TATGGTGTGGTTGTACGCATTTAGCATTAACGTCGACCACATAGATCTCGGAACGTTCACAGTCATCAAA
  TCTGTGATCTTTAAGATCGCGGACCATATAAACGTGATGACTCACACAATTTATTCTCCCGAGACTAATC
  TCCTCGTCTCTATTCTTCTGAATGCCTACACTGACTACCTCCAGAAATACGTAAATCCGTGGATCAAACA
  GACGATCACAGCTAACTTCTCACTCCTTCAGACTTACATTACCTTCACCAAGCAGTGCGCCTCTATCTTG
  GCAACCAAGTGCAATATCAATCTGGACAATTTGTTTATCTCAATGACAATCGGAACTGACAAGATTGTTA
  CAACATCTTTCTGCAGCTTCATAGCAACATGTCGAAACCTGGTGAGACAGCACGAGGAATTCAAGAAGAG
  TCTTAAGACCATTGAAACCAGTAAGACCACTCTCACCAACATGCTGCTTAATATTATAACAAGCGTGTCA
  TCATCCAAGGAGCTGTTGACAAACGAGGCGCTTCAAAAGTTCATTGACACCGTCCAGAGGATCTCACAGC
  ACGTTAACGAGACTTACCAGCTGATTAGTGTGAACCTGGAGAAATGTAAGATTTCAAACGATATCTTGAT
  TGAAAGTCTGAAGAAAACTATTTCAATTGTCGACGTGCTGTCCTCCGATGCCATCCTTAATACCAGTCTG
  ACTTCACGCTGCTTGGAAGCCGCTACCCTTGCTGTGTCAAACAACTCATTCACGATTCTCGAGATTAAGA
  AGGACGCAGTCGCCGTCTTCAAACCATTTATCACCCAGCTTTTCGAAAGCATGAAACCAACAACAAGCTT
  GTACAAAAAGCTGATGGCCACCCAGAAATTGACAACCGACCGCATCCCTTTCTTGGACATTTTCGACGAT
  AGGTACAACCTGGTCAGGCACGTGGAGAGGCAGCTGAACTGGTATGCTGCTTATGCTGAGGCAGCCCAAC
  AGGATCTGATTGCCCCACTTACTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101471 representing NP_050211
  Red=Cloning sites Green=Tags
  
  MTHDNRQLLPQETKLVTPNKDTLSSRYLHVLIIDNTLSTIEFVYMVVKAVLTQADTLKAFEQRKNRPTNR
  ILGLSTSVSKRYINIPKFSSSGANNSQGVKTLAAIRKFSLPDKNCFRNFLSFSTTLFKVPASIDIYFLFF
  TASTVSTMAARALDLEFILSSLKNSTSPESLLAATAKIEILSLAADSVTHNRVIAFINRLPPKKYHFDLI
  REYTVFYFLNSTTLTSENKLLSAELLHEELSQIRSSSSPGTELENLNNAEVLYVFDRILNSIKMLKNELS
  SPIGKLRVQPAANDPGTDKTIKYSKIQSIIQKVHSFTRENSLLANCRAVVELIDDLYRKLYSWFLHILTF
  EDIQFPGDTFLDRLLKMDYCFTYYPSSNRHLIDLFEKTLDNQTSTDIDKFFDTSGNSPELLYQKTFSLKI
  FSKNLTAQDNGLYIYPLLKTDLSILDFLGTENILFHRGLIYHILHQKTIPQERENDLNKINQFFATVIQQ
  VIETRSSCLPASLSRLLDTIFHFNRIGLNIETCRIYVEILSNHMATPDTQPIINTFTINLIHIVFTAHVF
  FICMENFSPTFLFYNRKKLILEQQRAILIIERNEYSTLWKQISDHIDCLFNISLSESFFKEYTKGGNEEH
  KQFLYKNLFEKWGDVFFPFTYSVTTSKNSTAHHITTLELRAICKEVYQSDSPEAYESLLPYSTHPAFKTL
  FIKIYVIPMVTYITNLTFDKLQSDYRLITLIHACKLLLPSQHLLLHYMVWLYAFSINVDHIDLGTFTVIK
  SVIFKIADHINVMTHTIYSPETNLLVSILLNAYTDYLQKYVNPWIKQTITANFSLLQTYITFTKQCASIL
  ATKCNINLDNLFISMTIGTDKIVTTSFCSFIATCRNLVRQHEEFKKSLKTIETSKTTLTNMLLNIITSVS
  SSKELLTNEALQKFIDTVQRISQHVNETYQLISVNLEKCKISNDILIESLKKTISIVDVLSSDAILNTSL
  TSRCLEAATLAVSNNSFTILEIKKDAVAVFKPFITQLFESMKPTTSLYKKLMATQKLTTDRIPFLDIFDD
  RYNLVRHVERQLNWYAAYAEAAQQDLIAPLTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 3246 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_000898.1, NP_050211
• RefSeq ORF: 3246 bp
• Locus ID: 1497032
• MW: 124.3 kDa