U28 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101469

Myc-DDK-tagged ORF clone of viral ORF for large ribonuclease reductase [Human herpesvirus 6], codon optimized for human cell expression, NP_050209

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101469 represents NCBI reference of NP_050209 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACGAAAAGAAAGACGGATTAACAAAGACTTCGGCTATAACAGGAAGTGCGTGTGCCATTATGAGG
  CCTCACAGAAAAGATTCTGTTACTCCCAGTATTCCTGTGCCAGCGTTCTGTATGAAAGGGTGCGCGACAT
  CGCTAAGATAATGGATCGCCTGGACAGTGGCCTGGATGCATGGTGCTTGCGGGATGCTATTATCTCTGTC
  CTGAGGGCGACTCATTGTGTCCCGCGCGTGGATCGGATGCTGGGTCGGTGGTACCTCAAGACGTCCATTT
  TTTATGATTTTTGTCCTGACGATCTGATCCTCAGTTGTCCCAACGTGATTATGCCAAACGTCCTTAACTT
  CGTGAAAAAATACAGAGACTTTATCCGAAGTGTACTGTATAAGGTCTCCGTTTCCTGGAAGAATCAGTAC
  ATGCCCGGAGTGCTGGGCGCGTCTCGCTTCCTGGAGGAGATATCCAACTCCCTCAACGGCGTCGAAGAGA
  GTATTCCATGTATATACCTGCGCATGTGCGCCACCCTGACAGAGATCGTGCTGCGCAATGGATATCTGAG
  GGAAATTTACCAGGAAAACCCTTACGTAATTTTTGAAGAACTCGCATTTAGCCTGTTTACCCAGAAGTGG
  GTACTGCCCTTTTCCTGTATGACTAATCTTGGCCTCGTAGAGAAAGCGAACTCCACCGTATTTGACGTTG
  CAATCTATAACACATGCCTGTATTCACTCGCCGATTTCATTACAGTCAACGGCGAGCACCTGTTCCCTGC
  CCTCCTCAATGGGTCCAATATTTCCATGAATGTCACAAGGTACCAGCAGGAGGCTAAGAATATTTTCGAG
  ATTCTTCTCTCTCAAATTCAAGTGGTAGAAAGGGACACCGATAAAACCGTCCAGCTTACTGTTTATGTAG
  AAGTGTGGCATGTCTCCGCTCTCACTTGGCTGGACCTGTACCAGGTGCTGCCCGAGACAAGCAGGGTCAC
  GTTCTGTCTGATTATCCCTGGCATCTTTATGGATCGCTATGAGCTGAAGCGCGCTCAGTGGTCCCTTTTC
  CATAAAAATATTGCTTTCGAGCTGGGCAAGTGTGACGAAGTTACCTTCTCTACAAAATATTTGGAATTCG
  AAAGAACAACTGACCACGCTAAAATCACTATGGCAAGCTTTGTAGAGAAGATTTGCAGATGTCTTAAACG
  CGGTCGGATGGGGCTTATTTTCCGAAAAAATGTTTACCAGTATTCTATGATCCCGCATGTCCCTCTGTAT
  TGCGGTGGCGATTTCCTTGATGTTCTTCCTGTTAGAGATGGCATTAATACATGCCTGCGAATGCTTCTGA
  ACGTGGTCCATTTTCTCGGCGACGAGGTGTCAGATGAGCTTACAGAGGAGATCGATTTTGTTCGGCTCCA
  ATGCAAGTTCTTTATGTTCAATGAACTGAGGAGAGTGGTTAGGAAAATGGTTTTGGTGGCCAATGCTGTG
  ATAGACTATGCGGTGGACAATAAAGACTTTCTGAGAGAGGGCATCGTCGACGGACGCTCTCTTGGAATCT
  GCATCACAGGGCTCCATTCAGTATTTATGACCGTCGGGCTGAGCTACGCACACCCTGATGCCTGTCGGCT
  CTACAGAATGATGTGTGAACATATTTATTATACATGCGTTCGAACATCAGTGGATTGCTGTATGAAAGGT
  GCCGAACCATGCAATCTGTTTGACCGCTCTAAGTATGCTTTGGGTATGCTGTACTTCGACCAGTTTGATA
  ATGTGGAATGCACTCTGCCTGAAGAGCTCTGGACTACACTGCGAAAGGATGTCCTGATGCATGGTGTCCG
  AAACATACATTTCACTGCCGGAACCGCCATGCAGAAAGAATTCGATATAATCAATTCTAGTGAATCCTTC
  TGGCCAATGGAGGACAACAAGATACTCCGAAGAAGCAATATCAAGGTGGTGATTGGGAAGGATGGACTTA
  ACGACGTCACCTCCGTCTACTCTAGCGAACTGAAGAGTCTGTACATCCCCGTTTACAATAACCTGTTGCT
  TAATAGGTTCAACAAGCATCAGCAATATCTTAAAACGGTCGGGTATCGGGTTCTGAACGTCGACACAAAC
  CTGTTTACGGACAAAGAACTTGACGACCTTGCTGTTTTCAAAGATGGCTTCAGTTATCCTCTTAATGACC
  TCATTGAGATGTATAAAAGCGGACTGCCCTTCCTGGACCAGGGCCAGGCTAACGTTTTCTATTTTAATGA
  CACCGTAAGTCTTAAACATCTGCTTCCTTTGCTCTACAAGACAGGTTTTAAGGTTGCCATGTATAAGGTT
  CTCTGCTCAAGCGAGATGTATAAGCATTTGGATCTGAGCAACCCACTCCCCTTGATCGGGAAGTGCTCTG
  ATGGAGTGGTCATGCACGTGAAAAATATTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101469 representing NP_050209
  Red=Cloning sites Green=Tags
  
  MKRKERRINKDFGYNRKCVCHYEASQKRFCYSQYSCASVLYERVRDIAKIMDRLDSGLDAWCLRDAIISV
  LRATHCVPRVDRMLGRWYLKTSIFYDFCPDDLILSCPNVIMPNVLNFVKKYRDFIRSVLYKVSVSWKNQY
  MPGVLGASRFLEEISNSLNGVEESIPCIYLRMCATLTEIVLRNGYLREIYQENPYVIFEELAFSLFTQKW
  VLPFSCMTNLGLVEKANSTVFDVAIYNTCLYSLADFITVNGEHLFPALLNGSNISMNVTRYQQEAKNIFE
  ILLSQIQVVERDTDKTVQLTVYVEVWHVSALTWLDLYQVLPETSRVTFCLIIPGIFMDRYELKRAQWSLF
  HKNIAFELGKCDEVTFSTKYLEFERTTDHAKITMASFVEKICRCLKRGRMGLIFRKNVYQYSMIPHVPLY
  CGGDFLDVLPVRDGINTCLRMLLNVVHFLGDEVSDELTEEIDFVRLQCKFFMFNELRRVVRKMVLVANAV
  IDYAVDNKDFLREGIVDGRSLGICITGLHSVFMTVGLSYAHPDACRLYRMMCEHIYYTCVRTSVDCCMKG
  AEPCNLFDRSKYALGMLYFDQFDNVECTLPEELWTTLRKDVLMHGVRNIHFTAGTAMQKEFDIINSSESF
  WPMEDNKILRRSNIKVVIGKDGLNDVTSVYSSELKSLYIPVYNNLLLNRFNKHQQYLKTVGYRVLNVDTN
  LFTDKELDDLAVFKDGFSYPLNDLIEMYKSGLPFLDQGQANVFYFNDTVSLKHLLPLLYKTGFKVAMYKV
  LCSSEMYKHLDLSNPLPLIGKCSDGVVMHVKNIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_000898.1, NP_050209
• RefSeq ORF: 2412 bp
• Locus ID: 1497030
• MW: 93.3 kDa