U11 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101454

Myc-DDK-tagged ORF clone of viral ORF for antigenic virion protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050192

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101454 represents NCBI reference of NP_050192 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGAAAGCGCAGTCAATTCCTTTTGCTTGGCTTGACCGAGACAAAGTCCAGAGACTGACAAACT
  TCCTTAGCAATCTGGAAAATCTCGAGAACGTGGACCTCAGAGAGCACCCCTACGTGACCAATAGCTGCGT
  TGTGCGAGAGGGCGAAGATGTGGACGAGCTTAAAACACTCTACAACACTTTCATTCTGTGGCTGATGTAC
  CACTACGTGCTGAGCAAACGCAAGCCAGACTACAATGCAATCTGGCAGGATATCACTAAGTTGCAGAACG
  TTGTGAATGAGTATCTGAAAAGCAAGGGCCTTAACAAAGGCAATTTTGAGAATATGTTCACCAACAAGGA
  GAAATTCGAGTCTCAGTTTAGTGATATCCATCGGGCTCTGCTCCGCTTGGGCAATTCTATCCGGTGGGGA
  TCCAACGTGCCGATCGATACCCCCTATGTGAATCTGACTGCTGAAGACTCTTCCGAAATCGAGAATAACC
  TGCAGGATGCAGAGAAAAATATGCTGTGGTATACAGTATACAACATCAACGACCCATGGGATGAGAACGG
  GTACCTGGTGACAAGCATTAACAAACTGGTGTACCTTGGGAAACTGTTTGTCACTCTCAACCAATCATGG
  AGCAAACTGGAGAAGGTCGCAATGTCACAGATTGTCACTACCCAGAATCATTTGAGTGGGCATCTGCGCA
  AGAATGAAAACTTCAACGCTGTGTATTCTCAGAGAGTCCTGCAGACCCCTCTGACAGGCCAACGGGTCGA
  GTCTTTTCTGAAAATTATCACCAGCGATTACGAAATTATTAAGAGCAGCCTGGAGTCTTATTCAGCATCA
  AAAGCCTTTAGCGTTCCGGAGAACGGCCCCCATTCCCTGATGGATTTCGCCTCCTTGGACGGGAGGATGC
  CAAGTGACTTGTCCCTGCCTTCCATCTCTATCGACACCAAGCGCCCTAGTGCCGACCTTGCCAGACTCAA
  AATCAGCCAGCCGAAATCACTTGATGCACCTCTGAAGACCCAGAGGAGACACAAGTTTCCTGAGTCCGAC
  AGCGTTGATAACGCCGGTGGCAAGATCTTGATAAAGAAAGAGACCCTTGGCGGACGCGATGTGAGGGCCA
  CGACCCCAGTGTCTAGCGTGTCCTTGATGTCCGGCGTCGAACCCCTGAGTAGCCTGACATCTACCAATCT
  GGACCTTCGAGACAAATCACACGGCAATTATAGAATCGGGCCTAGCGGGATACTTGACTTCGGGGTGAAG
  CTGCCAGCTGAGGCGCAAAGCAACACGGGCGACGTGGATTTGTTGCAGGATAAGACCAGTATTCGCAGCC
  CTTCTTCAGGCATCACTGATGTGGTGAATGGCCTGGCAAATCTCAATCTCCGCCAAAATAAAAGCGACGT
  GTCTCGGCCATGGTCCAAAAATACAGCCGCCAATGCGGACGTGTTTGATCCTGTTCACCGCCTGGTGAGT
  GAGCAGACCGGTACTCCGTTCGTGCTGAATAACTCAGATGTGGCTGGGTCCGAGGCAAAACTGACGACCC
  ATTCCACGGAGACCGGAGTCTCCCCTCATAACGTTAGTCTGATTAAGGATCTGAGGGATAAAGATGGATT
  TCGGAAGCAGAAAAAGCTGGACTTGCTCGGATCATGGACTAAAGAGAAAAACGACAAAGCCATAGTCCAT
  TCCAGAGAGGTCACTGGCGACTCCGGAGATGCAACGGAGACTGTAACTGCAAGGGATAGTCCAGTGCTGC
  GCAAAACCAAGCACGCTAATGATATTTTTGCTGGTTTGAACAAAAAGTATGCACGGGACGTCTCTCGGGG
  CGGTAAAGGCAATTCACGCGACCTGTATAGTGGGGGTAACGCAGAGAAAAAAGAGACCAGTGGGAAGTTT
  AATGTGGATAAGGAGATGACGCAGAATGAACAGGAACCTCTTCCCAACCTGATGGAGGCAGCTCGCAACG
  CCGGAGAGGAACAATACGTGCAGGCTGGCCTTGGTCAGCGCGTGAATAAAATACTGGCTGAGTTCACCAA
  TTTGATTAGCCTGGGGGAGAAAGGAATCCAGGATATTCTGCATAACCAATCTGGAACTGAACTGAAGCTG
  CCCACAGAGAATAAGTTGGGGAGGGAGAGCGAGGAGGCGAACGTCGAGAGGATACTTGAGGTAAGTGATC
  CCCAGAATCTGTTCAAGAACTTCAAGCTGCAGAATGACCTGGACTCAGTGCAGTCACCTTTCAGGCTCCC
  CAATGCTGACTTGAGCAGGGACTTGGACAGCGTCAGCTTCAAGGATGCACTGGATGTGAAACTGCCTGGC
  AATGGGGAGAGAGAAATAGATCTGGCTCTCCAAAAAGTTAAAGCTGGCGAGAGAGAAACATCAGATTTCA
  AGGTTGGTCAGGACGAAACTCTGATTCCCACGCAGTTGATGAAGGTGGAAACCCCGGAAGAGAAGGACGA
  TGTGATTGAAAAAATGGTGTTGAGAATACGCCAGGATGGGGAGACCGACGAAGAGACTGTCCCAGGCCCT
  GGGGTGGCAGAATCTCTTGGGATCGCAGCAAAAGACAAGAGCGTGATCGCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101454 representing NP_050192
  Red=Cloning sites Green=Tags
  
  MDLKAQSIPFAWLDRDKVQRLTNFLSNLENLENVDLREHPYVTNSCVVREGEDVDELKTLYNTFILWLMY
  HYVLSKRKPDYNAIWQDITKLQNVVNEYLKSKGLNKGNFENMFTNKEKFESQFSDIHRALLRLGNSIRWG
  SNVPIDTPYVNLTAEDSSEIENNLQDAEKNMLWYTVYNINDPWDENGYLVTSINKLVYLGKLFVTLNQSW
  SKLEKVAMSQIVTTQNHLSGHLRKNENFNAVYSQRVLQTPLTGQRVESFLKIITSDYEIIKSSLESYSAS
  KAFSVPENGPHSLMDFASLDGRMPSDLSLPSISIDTKRPSADLARLKISQPKSLDAPLKTQRRHKFPESD
  SVDNAGGKILIKKETLGGRDVRATTPVSSVSLMSGVEPLSSLTSTNLDLRDKSHGNYRIGPSGILDFGVK
  LPAEAQSNTGDVDLLQDKTSIRSPSSGITDVVNGLANLNLRQNKSDVSRPWSKNTAANADVFDPVHRLVS
  EQTGTPFVLNNSDVAGSEAKLTTHSTETGVSPHNVSLIKDLRDKDGFRKQKKLDLLGSWTKEKNDKAIVH
  SREVTGDSGDATETVTARDSPVLRKTKHANDIFAGLNKKYARDVSRGGKGNSRDLYSGGNAEKKETSGKF
  NVDKEMTQNEQEPLPNLMEAARNAGEEQYVQAGLGQRVNKILAEFTNLISLGEKGIQDILHNQSGTELKL
  PTENKLGRESEEANVERILEVSDPQNLFKNFKLQNDLDSVQSPFRLPNADLSRDLDSVSFKDALDVKLPG
  NGEREIDLALQKVKAGERETSDFKVGQDETLIPTQLMKVETPEEKDDVIEKMVLRIRQDGETDEETVPGP
  GVAESLGIAAKDKSVIAS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000898
• ORF Size: 2574 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_000898.1, NP_050192
• RefSeq ORF: 2574 bp
• Locus ID: 1497011
• MW: 95.6 kDa