U37 (NC_001664) Virus Tagged ORF Clone
CAT#: VC101385
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for nuclear egress lamina protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042930
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | U37 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC101385 represents NCBI reference of NP_042930 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGTGCATAAATCTCGCATAAGGAGGTCCAGGTCACTGTCAGTGACCCACCGCATCCAGAAAAGAC CTGATCACCGGGAAAAAACAAAACTGTACCTGCAACTGAAGTTGCACGACTTGCACACAGTGTTCAACCT GTTTCCCGAGTATGAACAAAAATTCTTGGCTATAATTAAATTGCCAATCACCGGAAAGGAGCCAATTGAT GTGCCGTTCTCACTCTCCAATCACCACCAGCACACATGTCTGGAATTTTCTCCGTATGCTAACGAGCAGA TCAGCAAAAGCGCATGTCTCCATTGTGAAAGCGTAAGTGTCCCCACCTCAAGTGACGCCATGGTGGCACA CCTTAATCAGGTCAACAACGTGATGCAAAACAGACTGTACTTTTATGGGTTCCGGAAGGACATGGAGCTG ATCCGGATGAGCGCCAAACAGCCTACAATTTTTCAGATCTTCTATATTGTGCACAACACAATTAATAATA TCTTTCCTATCATGTTCGAACGCAAGCAGAAGCTGGGCATGCACATTGTGTTTCAGAGCCGGACACTCCA CATTCCATGCGAGTGTATCAAGCAGATCGTTGCTGTTTCTTCTGGTTACAATGTGTACCTTGATATACTG CAGGAGTCTGTGATCCTCACCGTGCTCTGTGAAACACTGGATACCAATACTAACATCCACATTGATATCG GGATGTTGCAGAAGAAGCTGGAAGAAATGGATATCCCTAACGAGATCTCAGATAGATTGGAGAAGTACAA GGGACACCTGATAGGCTTCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101385 representing NP_042930
Red=Cloning sites Green=Tags MTVHKSRIRRSRSLSVTHRIQKRPDHREKTKLYLQLKLHDLHTVFNLFPEYEQKFLAIIKLPITGKEPID VPFSLSNHHQHTCLEFSPYANEQISKSACLHCESVSVPTSSDAMVAHLNQVNNVMQNRLYFYGFRKDMEL IRMSAKQPTIFQIFYIVHNTINNIFPIMFERKQKLGMHIVFQSRTLHIPCECIKQIVAVSSGYNVYLDIL QESVILTVLCETLDTNTNIHIDIGMLQKKLEEMDIPNEISDRLEKYKGHLIGFH TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001664 |
ORF Size | 792 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_001664.2, NP_042930 |
RefSeq ORF | 792 bp |
Locus ID | 1487915 |
MW | 30.8 kDa |
Documents
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Resources
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