VP29 precursor (NC_001943) Virus Tagged ORF Clone

CAT#: VC100534

Myc-DDK-tagged ORF clone of viral ORF for capsid precursor protein [Human astrovirus], codon optimized for human cell expression, NP_059444

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP29 precursor
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100534 represents NCBI reference of NP_059444 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAAAGTCTAACAAACAGGTGACCGTCGAGGTGTCAAACAACGGCAGGAGCAGAAGCAAATCAC
  GGGCGCGGTCTCAGTCACGCGGTCGGGACAAGTCTGTCAAGATTACCGTAAACAGTCGGAATAGGGCACG
  CCGGCAGCCTGGCAGAGATAAACGCCAGAGCTCACAGCGGGTTAGGAATATTGTCAACAAGCAGTTGAGA
  AAGCAGGGAGTCACCGGGCCAAAGCCTGCTATCTGCCAGCGGGCCACCGCTACCCTGGGCACAGTCGGCT
  CAAATACCTCTGGAACCACAGAGATCGAGGCCTGCATCCTGCTCAATCCAGTGCTGGTTAAGGATGCCAC
  AGGGTCCACCCAGTTCGGCCCAGTACAGGCTCTGGGTGCCCAATACAGCATGTGGAAATTGAAGTACCTG
  AACGTTAAACTCACTTCCATGGTTGGGGCCTCCGCCGTAAACGGCACTGTCAGCGGAGTGAGTCTGAATC
  CAACCACAACTCCCACTTCCACTAGCTGGTCTGGCCTTGGAGCACGGAAACACCTTGACGTCACAGTCGG
  CAAAAATGCGACGTTCAAACTGAAACCAAGCGATCTGGGCGGTCCCCGCGACGGCTGGTGGCTGACCAAT
  ACAAACGATAATGCATCAGACACCCTTGGTCCATCCATAGAAATTCATACCCTCGGCCGGACAATGTCTA
  GTTATAAAAATGAACAGTTCACTGGGGGATTGTTCCTCGTGGAACTGGCAAGTGAGTGGTGCTTTACGGG
  CTATGCCGCTAATCCTAATCTCGTGAATCTGGTAAAGAGCACCGACAACCAGGTGAGCGTGACATTCGAA
  GGGTCCGCGGGGTCCCCACTGATCATGAACGTCCCTGAAGGATCTCACTTTGCACGGACCGTTTTGGCCA
  GGAGTACGACCCCTACCACACTGGCCCGGGCGGGTGAAAGAACCACCTCAGACACTGTTTGGCAAGTGCT
  CAATACCGCCGTGTCCGCTGCGGAATTGGTCACCCCACCTCCCTTTAATTGGCTGGTCAAAGGCGGCTGG
  TGGTTCGTTAAGCTGATAGCCGGAAGGACCAGAACCGGATCCCGCAGTTTCTATGTCTACCCAAGCTATC
  AGGACGCACTTTCCAACAAGCCAGCTTTGTGCACGGGCAGTACACCCGGCGGCATGAGAACCCGCAATCC
  TGTGACAACCACACTGCAGTTCACCCAGATGAACCAGCCCAGTCTCGGGCACGGGGAGGCCCCTGCAGCC
  TTTGGGCGCTCTATCCCGGCCCCCGGGGAAGAATTCAAGGTCGTCTTGACCTTTGGCGCCCCCATGTCTC
  CGAATGCTAACAATAAACAGACTTGGGTGAACAAGCCTCTGGATGCCCCTAGCGGCCATTATAACGTGAA
  AATTGCGAAGGATGTTGACCACTACCTCACCATGCAAGGCTTTACTAGCATCGCTTCCGTGGACTGGTAC
  ACCATTGATTTTCAGCCAAGCGAGGCTCCTGCGCCCATCCAGGGGCTGCAGGTTCTGGTGAACTCCAGCA
  AGAAAGCTGATGTGTACGCAATTAAACAGTTTGTCACTGCACAAACTAACAACAAACACCAGGTCACTAG
  CCTCTTTCTCGTCAAGGTAACTACCGGGTTTCAGGTGAATAACTACCTGAGCTACTTTTATCGCGCAAGT
  GCAACCGGGGATGCCACAACTAATCTGCTCGTGAGGGGCGACACATACACCGCCGGGATCTCATTTACCC
  AGGGTGGATGGTACCTGCTGACTAACACAAGTATCGTCGACGGCGCGATGCCCCCTGGCTGGGTGTGGAA
  TAACGTTGAGCTCAAAACCAATACAGCTTACCACATGGATAAAGGTCTCGTACATCTGATTATGCCTCTG
  CCTGAGTCCACACAGATGTGCTACGAAATGCTGACCAGCATCCCCAGGTCAAGGGCGAGCGGACATGGAT
  ACGAGTCCGACAACACCGAGTATCTGGACGCACCAGATTCAGCCGACCAATTTAAGGAGGATATTGAGAC
  CGACACTGACATTGAGAGCACAGAGGACGAGGACGAGGCCGATCGATTTGACATCATTGACACCTCCGAT
  GAGGAGGACGAAAACGAGACAGACCGGGTGACTTTGCTGAGCACACTGGTTAACCAGGGGATGACTATGA
  CCCGAGCCACGCGGATTGCTAGGCGCGCCTTCCCTACGTTGAGCGACCGCATCAAGCGGGGTGTTTACAT
  GGACCTCCTCGTGAGCGGCGCATCCCCTGGGAACGCCTGGTCCCACGCATGCGAGGAAGCTAGGAAAGCC
  GCCGGCGAGATAAATCCTTGTACCAGCGGTTCCAGAGGTCACGCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100534 representing NP_059444
  Red=Cloning sites Green=Tags
  
  MASKSNKQVTVEVSNNGRSRSKSRARSQSRGRDKSVKITVNSRNRARRQPGRDKRQSSQRVRNIVNKQLR
  KQGVTGPKPAICQRATATLGTVGSNTSGTTEIEACILLNPVLVKDATGSTQFGPVQALGAQYSMWKLKYL
  NVKLTSMVGASAVNGTVSGVSLNPTTTPTSTSWSGLGARKHLDVTVGKNATFKLKPSDLGGPRDGWWLTN
  TNDNASDTLGPSIEIHTLGRTMSSYKNEQFTGGLFLVELASEWCFTGYAANPNLVNLVKSTDNQVSVTFE
  GSAGSPLIMNVPEGSHFARTVLARSTTPTTLARAGERTTSDTVWQVLNTAVSAAELVTPPPFNWLVKGGW
  WFVKLIAGRTRTGSRSFYVYPSYQDALSNKPALCTGSTPGGMRTRNPVTTTLQFTQMNQPSLGHGEAPAA
  FGRSIPAPGEEFKVVLTFGAPMSPNANNKQTWVNKPLDAPSGHYNVKIAKDVDHYLTMQGFTSIASVDWY
  TIDFQPSEAPAPIQGLQVLVNSSKKADVYAIKQFVTAQTNNKHQVTSLFLVKVTTGFQVNNYLSYFYRAS
  ATGDATTNLLVRGDTYTAGISFTQGGWYLLTNTSIVDGAMPPGWVWNNVELKTNTAYHMDKGLVHLIMPL
  PESTQMCYEMLTSIPRSRASGHGYESDNTEYLDAPDSADQFKEDIETDTDIESTEDEDEADRFDIIDTSD
  EEDENETDRVTLLSTLVNQGMTMTRATRIARRAFPTLSDRIKRGVYMDLLVSGASPGNAWSHACEEARKA
  AGEINPCTSGSRGHAE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001943
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001943.1, NP_059444
• RefSeq ORF: 2358 bp
• Locus ID: 1494461
• MW: 85.5 kDa